Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
Environ Sci Technol. 2024 Jan 9;58(1):603-616. doi: 10.1021/acs.est.3c08881. Epub 2023 Dec 18.
The mechanisms of the SL2-mediated microbial community on phosphorus solubilization and Pb stabilization were investigated through a 90-day soil experiment. In the treatments inoculated with SL2, the amount of SL2-GFP remained at 77.8%-138.6% of the initial inoculation amount after 90 days, and the available phosphorus (AP) content increased 21.7%-40.8% while EDTA-Pb decreased 29.9%-43.2% compared with CK treatment. SEM-EDS results showed that SL2 changed the agglomeration degree of microaggregates and promoted the combination of Pb with C and O elements. These phenomena were enhanced when applied with Ca(PO). Microbial community analysis showed that SL2 improved soil microbial activity, in which the fungi absolute abundance increased about 15 times within 90 days. Correlation analyses and a partial least-squares path model showed that the activation of , , , and in a fungal community increased the content of oxalate and AP, which directly decreased EDTA-Pb content, while the change of , , , and in the bacterial community regulated Fe/Mn/S/N cycle-related functions, thus promoting the conversion of Pb to oxidizable state. Our findings highlight that SL2 enhanced the microbial-induced phosphate precipitation process by activating soil microbial communities and regulating their ecological functions.
通过为期 90 天的土壤实验，研究了 SL2 介导的微生物群落对磷溶解释放和 Pb 稳定化的作用机制。在接种 SL2 的处理中，90 天后 SL2-GFP 的数量保持在初始接种量的 77.8%-138.6%，有效磷（AP）含量增加了 21.7%-40.8%，而 EDTA-Pb 则减少了 29.9%-43.2%，与 CK 处理相比。SEM-EDS 结果表明，SL2 改变了微团聚体的团聚程度，促进了 Pb 与 C 和 O 元素的结合。当与 Ca(PO)一起应用时，这些现象得到了增强。微生物群落分析表明，SL2 提高了土壤微生物活性，其中真菌绝对丰度在 90 天内增加了约 15 倍。相关分析和偏最小二乘路径模型表明，真菌群落中 、 、 、 和 的激活增加了草酸盐和 AP 的含量，这直接降低了 EDTA-Pb 的含量，而细菌群落中 、 、 、 和 的变化调节了 Fe/Mn/S/N 循环相关功能，从而促进了 Pb 向可氧化状态的转化。我们的研究结果强调了 SL2 通过激活土壤微生物群落并调节其生态功能，增强了微生物诱导的磷酸盐沉淀过程。
