Portell-Buj Elena, González-Criollo Cecibel, López-Gavín Alexandre, Fernández-Pittol Mariana, Busquets Maria Antònia, Estelrich Joan, Garrigó Montserrat, Rubio Marc, Tudó Griselda, Gonzalez-Martin Julian
Departament de Fonaments Clínics, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, c/Casanova 143, 08036 Barcelona, Spain.
ISGlobal Barcelona, Institute for Global Health, c/Rosselló 132, 08036 Barcelona, Spain.
Antibiotics (Basel). 2022 Apr 27;11(5):589. doi: 10.3390/antibiotics11050589.
Nontuberculous mycobacteria (NTM) cause lung infections in patients with underlying pulmonary diseases (PD). The - complex (MAC) is the most frequently involved NTM. The MAC-PD treatment is based on the administration of several antibiotics for long periods of time. Nonetheless, treatment outcomes remain very poor. Among the factors involved is the ability of MAC isolates to form biofilm. The aim of the study was to assess the in vitro activity of different antibiotics and potential antibiofilm agents (PAAs) against MAC biofilm. Four antibiotics and six PAAs, alone and/or in combination, were tested against planktonic forms of 11 MAC clinical isolates. Biofilm was produced after 4 weeks of incubation and analyzed with the crystal violet assay. The antibiotics and PAAs were tested by measuring the absorbance (minimum biofilm inhibition concentrations, MBICs) and by performing subcultures (minimum biofilm eradication concentrations, MBECs). The clarithromycin/amikacin and clarithromycin/ethambutol combinations were synergistic, decreasing the MBECs values compared to the individual antibiotics. The amikacin/moxifloxacin combination showed indifference. The MBIC values decreased significantly when PAAs were added to the antibiotic combinations. These results suggest that antibiotic combinations should be further studied to establish their antibiofilm activity. Moreover, PAAs could act against the biofilm matrix, facilitating the activity of antibiotics.
非结核分枝杆菌（NTM）可导致患有基础肺部疾病（PD）的患者发生肺部感染。鸟分枝杆菌复合群（MAC）是最常涉及的NTM。MAC-PD的治疗基于长时间使用多种抗生素。尽管如此，治疗效果仍然很差。其中涉及的因素包括MAC分离株形成生物膜的能力。本研究的目的是评估不同抗生素和潜在抗生物膜剂（PAA）对MAC生物膜的体外活性。单独和/或联合使用四种抗生素和六种PAA，对11株MAC临床分离株的浮游形式进行测试。孵育4周后产生生物膜，并用结晶紫测定法进行分析。通过测量吸光度（最低生物膜抑制浓度，MBIC）和进行传代培养（最低生物膜根除浓度，MBEC）来测试抗生素和PAA。克拉霉素/阿米卡星和克拉霉素/乙胺丁醇组合具有协同作用，与单独使用抗生素相比，降低了MBEC值。阿米卡星/莫西沙星组合无明显作用。当将PAA添加到抗生素组合中时，MBIC值显著降低。这些结果表明，应进一步研究抗生素组合以确定其抗生物膜活性。此外，PAA可作用于生物膜基质，促进抗生素的活性。
