Ugrayová Simona, Švec Peter, Hric Ivan, Šardzíková Sára, Kubáňová Libuša, Penesová Adela, Adamčáková Jaroslava, Pačesová Petra, Horáková Júlia, Kolenová Alexandra, Šoltys Katarína, Kolisek Martin, Bielik Viktor
Department of Biological and Medical Science, Faculty of Physical Education and Sport, Comenius University in Bratislava, 814 69 Bratislava, Slovakia.
Department of Pediatric Hematology and Oncology, Comenius University and National Institute of Children's Diseases, Limbova 1, 833 40 Bratislava, Slovakia.
Biology (Basel). 2022 May 21;11(5):785. doi: 10.3390/biology11050785.
Gut microbiome impairment is a serious side effect of cancer treatment. The aim of this study was to identify the effects of hematopoietic stem cell transplantation (HSCT) treatment on gut microbiota composition in children with acute lymphoblastic leukemia (ALL). Fecal microbiotas were categorized using specific primers targeting the V1-V3 region of 16S rDNA in eligible pediatric ALL patients after HSCT (n = 16) and in healthy controls (Ctrl, n = 13). An intra-hospital exercise program was also organized for child patients during HSCT treatment. Significant differences in gut microbiota composition were observed between ALL HSCT and Ctrl with further negative effects. Plasma C-reactive protein correlated positively with the pathogenic bacteria spp. and negatively with beneficial bacteria spp. or spp., respectively (rs = 0.511, = 0.05; rs = -0.541, = 0.04; rs = -0.738, = 0.02). Bacterial alpha diversity correlated with the exercise training characteristics. Therefore, specific changes in the microbiota of children were associated with systemic inflammation or the ability to exercise physically during HSCT treatment.
肠道微生物群受损是癌症治疗的一种严重副作用。本研究的目的是确定造血干细胞移植（HSCT）治疗对急性淋巴细胞白血病（ALL）儿童肠道微生物群组成的影响。在接受HSCT治疗的合格儿科ALL患者（n = 16）和健康对照（Ctrl，n = 13）中，使用靶向16S rDNA V1-V3区域的特异性引物对粪便微生物群进行分类。在HSCT治疗期间还为儿童患者组织了一项院内锻炼计划。观察到ALL HSCT组和Ctrl组之间肠道微生物群组成存在显著差异，且有进一步的负面影响。血浆C反应蛋白分别与病原菌属呈正相关，与有益菌属或菌属呈负相关（rs = 0.511，P = 0.05；rs = -0.541，P = 0.04；rs = -0.738，P = 0.02）。细菌α多样性与运动训练特征相关。因此，儿童微生物群的特定变化与HSCT治疗期间的全身炎症或身体运动能力有关。
