Ho-Plágaro Ailec, Rodríguez-Díaz Cristina, Santiago-Fernández Concepción, López-Gómez Carlos, García-Serrano Sara, Martín-Reyes Flores, Rodríguez-Pacheco Francisca, Rodríguez-Cañete Alberto, Alcaín-Martínez Guillermo, Vázquez-Pedreño Luis, Valdés Sergio, Garrido-Sánchez Lourdes, García-Fuentes Eduardo
Instituto de Investigación Biomédica de Málaga-IBIMA, 29010 Malaga, Spain.
Unidad de Gestión Clínica de Aparato Digestivo, Hospital Universitario Virgen de la Victoria, 29010 Malaga, Spain.
Biomedicines. 2022 Apr 29;10(5):1024. doi: 10.3390/biomedicines10051024.
Little is known about the relation between morbid obesity and duodenal transcriptomic changes. We aimed to identify intestinal genes that may be associated with the development of obesity regardless of the degree of insulin resistance (IR) of patients.
Duodenal samples were assessed by microarray in three groups of women: non-obese women and women with morbid obesity with low and high IR.
We identified differentially expressed genes (DEGs) associated with morbid obesity, regardless of IR degree, related to digestion and lipid metabolism, defense response and inflammatory processes, maintenance of the gastrointestinal epithelium, wound healing and homeostasis, and the development of gastrointestinal cancer. However, other DEGs depended on the IR degree. We mainly found an upregulation of genes involved in the response to external organisms, hypoxia, and wound healing functions in women with morbid obesity and low IR.
Regardless of the degree of IR, morbid obesity is associated with an altered expression of genes related to intestinal defenses, antimicrobial and immune responses, and gastrointestinal cancer. Our data also suggest a deficient duodenal immune and antimicrobial response in women with high IR.
关于病态肥胖与十二指肠转录组变化之间的关系，我们知之甚少。我们旨在确定无论患者胰岛素抵抗（IR）程度如何，可能与肥胖发生发展相关的肠道基因。
通过微阵列对三组女性的十二指肠样本进行评估：非肥胖女性以及IR程度低和高的病态肥胖女性。
我们鉴定出与病态肥胖相关的差异表达基因（DEG），无论IR程度如何，这些基因都与消化和脂质代谢、防御反应和炎症过程、胃肠道上皮的维持、伤口愈合和内稳态以及胃肠道癌症的发生发展有关。然而，其他DEG则取决于IR程度。我们主要发现，IR程度低的病态肥胖女性中，参与对外界生物体反应、缺氧和伤口愈合功能的基因上调。
无论IR程度如何，病态肥胖都与肠道防御、抗菌和免疫反应以及胃肠道癌症相关基因的表达改变有关。我们的数据还表明，IR程度高的女性十二指肠免疫和抗菌反应存在缺陷。
