Selvaraju Vaithinathan, Babu Jeganathan R, Geetha Thangiah
Department of Nutrition, Dietetics and Hospitality Management, Auburn University, Auburn, AL 36849, USA.
Boshell Diabetes and Metabolic Diseases Research Program, Auburn University, Auburn, AL 36849, USA.
Diagnostics (Basel). 2022 May 3;12(5):1130. doi: 10.3390/diagnostics12051130.
Obesity is linked with higher inflammatory markers and is characterized by chronic low-grade inflammation. Neurotrophins brain-derived neurotrophic factor (BDNF) and β-nerve growth factor (β-NGF), in addition to their neuronal functions, act on several immune cells and have been recently designated as metabokines due to their regulatory role in energy homeostasis and food intake. The current study evaluates the salivary BDNF and β-NGF and their association with anthropometric measurement, blood pressure, and salivary insulin in children. Anthropometric measurements and saliva samples were obtained from 76 children, aged 6-10 years. Multiplex analysis was carried out for the salivary analysis of BDNF, NGF, and insulin by human magnetic Luminex performance assay. Statistical analysis was performed to analyze the best fit diagnostic value for biomarkers and the relationship of the neurotrophic levels of BDNF and NGF with obesity measures and blood pressure. Salivary BDNF and β-NGF showed a significantly higher concentration in obese children than normal-weight children. Both neurotrophins are positively associated with obesity anthropometric measures, blood pressure, and salivary insulin. Multinominal regression analysis reported a significant association between salivary BDNF, β-NGF, insulin, and systolic pressure adjusted for age, gender, income, and maternal education. The salivary concentration of BDNF and NGF was higher in obese children, and it is positively associated with anthropometric measures, suggesting that neurotrophins can be used as a non-invasive predictor of obesity-related complications in children.
肥胖与较高的炎症标志物相关，其特征为慢性低度炎症。神经营养因子脑源性神经营养因子（BDNF）和β-神经生长因子（β-NGF），除了具有神经元功能外，还作用于多种免疫细胞，并且由于它们在能量稳态和食物摄入中的调节作用，最近被指定为代谢因子。本研究评估了儿童唾液中的BDNF和β-NGF及其与人体测量、血压和唾液胰岛素的关系。从76名6至10岁的儿童中获取了人体测量数据和唾液样本。通过人磁性Luminex性能测定法对唾液中的BDNF、NGF和胰岛素进行多重分析。进行统计分析以分析生物标志物的最佳拟合诊断值以及BDNF和NGF的神经营养水平与肥胖测量值和血压之间的关系。肥胖儿童唾液中的BDNF和β-NGF浓度明显高于正常体重儿童。这两种神经营养因子均与肥胖人体测量指标、血压和唾液胰岛素呈正相关。多项回归分析报告称，在调整了年龄、性别、收入和母亲教育程度后，唾液中的BDNF 、β-NGF、胰岛素与收缩压之间存在显著关联。肥胖儿童唾液中BDNF和NGF的浓度较高，且与人体测量指标呈正相关，这表明神经营养因子可作为儿童肥胖相关并发症的非侵入性预测指标。
