Park Jung Hyun, Cho Sun-Jung, Jo Chulman, Park Moon Ho, Han Changsu, Kim Eun-Joo, Huh Gi Yeong, Koh Young Ho
Department of Chronic Disease Convergence Research, Division of Brain Disease Research, Korea National Institute of Health, 187 Osongsaengmyeong2-ro, Osong-eup, Heungdeok-gu, Cheongju-si 28159, Korea.
Departments of Neurology, Korea University Medical College, Ansan Hospital, 123 Jeokgeum-ro, Danwon-gu, Ansan-si 15355, Korea.
J Pers Med. 2022 May 19;12(5):827. doi: 10.3390/jpm12050827.
Tissue inhibitor of metalloproteinase-3 (TIMP-3) is a component of the extracellular environment and is suggested to play an indirect role in regulating Aβ production and the pathophysiology of Aβ deposition in brains. However, studies on the amount of TIMP-3 in bodily fluids of Alzheimer's disease (AD) patients have not been conducted. Here, we investigated the relationship between fluid TIMP-3 levels and AD pathology. We first showed that the fluid levels of TIMP-3 were lower in AD dementia patients compared with in non-AD patients. ELISA results revealed that plasma levels of TIMP-3 in 65 patients with AD were significantly lower than those in 115 healthy control subjects and 71 mild cognitive impairment (MCI) subjects. Furthermore, we found that cerebrospinal fluid (CSF) level of TIMP-3 was decreased in AD compared with that in healthy control. These data suggest that fluid TIMP-3 levels negatively correlated with progress of cognitive decline. Collectively, our study suggests that alterations of fluid TIMP-3 levels might be associated with AD pathology.
金属蛋白酶组织抑制剂-3（TIMP-3）是细胞外环境的一个组成部分，据认为在调节β淀粉样蛋白（Aβ）生成以及Aβ在大脑中沉积的病理生理学过程中发挥间接作用。然而，尚未对阿尔茨海默病（AD）患者体液中的TIMP-3含量进行研究。在此，我们调查了体液TIMP-3水平与AD病理学之间的关系。我们首先表明，与非AD患者相比，AD痴呆患者的体液TIMP-3水平较低。酶联免疫吸附测定（ELISA）结果显示，65例AD患者的血浆TIMP-3水平显著低于115例健康对照者和71例轻度认知障碍（MCI）患者。此外，我们发现与健康对照相比，AD患者脑脊液（CSF）中的TIMP-3水平降低。这些数据表明，体液TIMP-3水平与认知衰退进程呈负相关。总体而言，我们的研究表明，体液TIMP-3水平的改变可能与AD病理学相关。
