Matsyura Oksana, Besh Lesya, Kens Olena, Kosorinová Dana, Volkovová Katarína, Vari Sandor G
Department of Pediatrics №2, Danylo Halytsky Lviv National Medical University, 79010 Lviv, Ukraine.
Institute of Hereditary Pathology of National Academy of Medical Sciences of Ukraine, 79000 Lviv, Ukraine.
Life (Basel). 2022 Apr 19;12(5):612. doi: 10.3390/life12050612.
Cow’s milk allergy (CMA) is one of the most frequent types of food allergy. The aim of the study was to investigate whether IL-13 R130Q and IL-4 T589C polymorphisms are associated with the risk of CMA in young Ukrainian children. A total of 120 children (age range: 1−3 years) participated in the study and were divided into two groups: CMA (n = 60) and healthy controls (CNT, n = 60). The CMA group had two subgroups: specific oral tolerance induction (SOTI, n = 30) and milk elimination diet (MED, n = 30). The CNT group had two subgroups: positive family history of allergy (+FHA, n = 24) and negative family history of allergy (−FHA, n = 36). In the CMA group, molecular genetic testing of CC, CT, and TT genotypes of single nucleotide IL-4 T589C gene polymorphisms showed significantly higher rates of the CC genotype compared to healthy controls (92.2% vs. 58.8%; p < 0.01). In the CMA group, molecular genetic testing of GG, GA, and AA genotypes of single nucleotide IL-13 R130Q gene polymorphisms showed significantly higher rates of GA and AA polymorphic locus genotypes compared to healthy controls (43.5% vs. 22.4%, p < 0.05 and 8.7% vs. 0%, p < 0.05, respectively). In future studies, the genotypic and allelic distribution of these polymorphic variants will be determined in children with CMA and healthy children.
牛奶过敏（CMA）是最常见的食物过敏类型之一。本研究的目的是调查IL-13 R130Q和IL-4 T589C基因多态性是否与乌克兰幼儿患CMA的风险相关。共有120名儿童（年龄范围：1 - 3岁）参与了该研究，并被分为两组：CMA组（n = 60）和健康对照组（CNT，n = 60）。CMA组有两个亚组：特异性口服耐受诱导组（SOTI，n = 30）和牛奶排除饮食组（MED，n = 30）。CNT组有两个亚组：有过敏家族史阳性组（+FHA，n = 24）和无过敏家族史阴性组（−FHA，n = 36）。在CMA组中，单核苷酸IL-4 T589C基因多态性的CC、CT和TT基因型的分子遗传学检测显示，与健康对照组相比，CC基因型的比例显著更高（92.2%对58.8%；p < 0.01）。在CMA组中，单核苷酸IL-13 R130Q基因多态性的GG、GA和AA基因型的分子遗传学检测显示，与健康对照组相比，GA和AA多态性位点基因型的比例显著更高（分别为43.5%对22.4%，p < 0.05和8.7%对0%，p < 0.05）。在未来的研究中，将确定这些多态性变体在CMA儿童和健康儿童中的基因型和等位基因分布。
