Rotunno Silvia, Cocozza Claudia, Pantaleo Vitantonio, Leonetti Paola, Bertoldi Loris, Valle Giorgio, Accotto Gian Paolo, Loreto Francesco, Scippa Gabriella Stefania, Miozzi Laura
Department of Biosciences and Territory, University of Molise, Contrada Fonte Lappone, 86090 Pesche, Italy.
National Research Council of Italy, Institute for Sustainable Plant Protection (CNR-IPSP), Strada delle Cacce 73, 10135 Torino, Italy.
Life (Basel). 2022 Apr 27;12(5):651. doi: 10.3390/life12050651.
MicroRNAs (miRNAs) are a class of non-coding molecules involved in the regulation of a variety of biological processes. They have been identified and characterized in several plant species, but only limited data are available for L., one of the most promising bioenergy crops. Here we identified, for the first time, conserved and novel miRNAs together with their targets, through a combined analysis of high-throughput sequencing of small RNAs, transcriptome and degradome data. A total of 134 conserved miRNAs, belonging to 45 families, and 27 novel miRNA candidates were identified, along with the corresponding primary and precursor miRNA sequences. A total of 96 targets, 69 for known miRNAs and 27 for novel miRNA candidates, were also identified by degradome analysis and selected slice sites were validated by 5'-RACE. The identified set of conserved and novel candidate miRNAs, together with their targets, extends our knowledge about miRNAs in monocots and pave the way to further investigations on miRNAs-mediated regulatory processes in , Poaceae and other bioenergy crops.
微小RNA（miRNA）是一类参与多种生物过程调控的非编码分子。它们已在多种植物物种中被鉴定和表征，但对于最具潜力的生物能源作物之一柳枝稷而言，相关数据仍然有限。在此，我们通过对小RNA高通量测序、转录组和降解组数据的联合分析，首次鉴定出了保守和新的miRNA及其靶标。共鉴定出属于45个家族的134个保守miRNA和27个新的miRNA候选物，以及相应的初级和前体miRNA序列。通过降解组分析还鉴定出了总共96个靶标，其中已知miRNA的靶标有69个，新miRNA候选物的靶标有27个，并通过5'-RACE验证了选定的切割位点。所鉴定出的保守和新候选miRNA及其靶标，扩展了我们对单子叶植物中miRNA的认识，并为进一步研究柳枝稷、禾本科及其他生物能源作物中miRNA介导的调控过程铺平了道路。
