Kercheva Maria, Ryabov Vyacheslav, Trusov Andrey, Stepanov Ivan, Kzhyshkowska Julia
Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 111a Kievskaya Street, 634012 Tomsk, Russia.
Central Research Laboratory, Siberian State Medical University, 2 Moscovsky Trakt, 634055 Tomsk, Russia.
Life (Basel). 2022 May 1;12(5):673. doi: 10.3390/life12050673.
Myocardial ischemia triggers neurohumoral activation of the cardiosplenic axis. In rodents, adverse outcomes occur upon prolonged entrance of mononuclear cells from the spleen into myocardial tissue. The purpose of this study is to assess the features of spleen structure in patients with fatal myocardial infarction (MI), the dynamics of macrophage infiltration of the spleen and its relationship with cardiac macrophage infiltration and unfavorable outcomes. Using immunohistochemistry techniques, we analyzed the macrophage infiltration of the spleen and myocardium sections collected from patients ( = 30) with fatal MI. The spleen of the patients was decreased and showed a predominance of red pulp with a high concentration of CD68+ and stabilin-1+ cells. The white pulp contained many medium and small follicles and a lower concentration of CD68+ and stabilin-1+ cells, which was comparable to that in the infarct area of the myocardium. The concentration of CD68+ and stabilin-1+ cells increased in the myocardium in the late period of MI, but did not show any dynamics in the spleen. A high number of CD68+ cells in the red pulp and reduced concentration of stabilin-1+ cells in the white pulp were associated with unfavorable post-infarction outcomes. These fundamental findings could be a basis for the development of new personalized therapeutic and diagnostic approaches for the treatment of MI and its complications.
心肌缺血会引发心脾轴的神经体液激活。在啮齿动物中，当脾脏中的单核细胞长时间进入心肌组织时，会出现不良后果。本研究的目的是评估致命性心肌梗死（MI）患者脾脏结构的特征、脾脏巨噬细胞浸润的动态变化及其与心脏巨噬细胞浸润和不良后果的关系。我们使用免疫组织化学技术，分析了从30例致命性MI患者收集的脾脏和心肌切片中的巨噬细胞浸润情况。患者的脾脏缩小，以红髓为主，CD68+和稳定素-1+细胞浓度较高。白髓中有许多中小滤泡，CD68+和稳定素-1+细胞浓度较低，这与心肌梗死区域的情况相当。MI后期心肌中CD68+和稳定素-1+细胞浓度增加，但脾脏中未显示出任何动态变化。红髓中大量的CD68+细胞和白髓中稳定素-1+细胞浓度降低与梗死后不良后果相关。这些重要发现可为开发治疗MI及其并发症的新的个性化治疗和诊断方法奠定基础。
