Diaz-Hernandez Alejandro, Gonzalez-Vazquez Maria Cristina, Arce-Fonseca Minerva, Rodríguez-Morales Olivia, Cedillo-Ramirez Maria Lilia, Carabarin-Lima Alejandro
Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, 14 Sury Avenida San Claudio, Ciudad Universitaria, Puebla 72570, Mexico.
Herbario y Jardín Botánico Universitario, Benemérita Universidad Autónoma de Puebla, Ciudad Universitaria, Puebla 72570, Mexico.
Life (Basel). 2022 May 18;12(5):746. doi: 10.3390/life12050746.
There is currently no vaccine against American trypanosomiasis, caused by the parasite . This is due to the genomic variation observed in the six DTUs of . This work aims to propose a consensus sequence of the enolase protein from different strains of and mainly evaluate its immunogenic properties at the bioinformatic level. From specialized databases, 15 sequences of the enolase gene were aligned to obtain a consensus sequence, where this sequence was modeled and then evaluated and validated through different bioinformatic programs to learn their immunogenic potential. Finally, chimeric peptides were designed with the most representative epitopes. The results showed high immunogenic potential with six epitopes for MHC-I, and seven epitopes for MHC-II, all of which were highly representative of the enolase present in strains from the American continent as well as five epitopes for B cells. Regarding the computational modeling, molecular docking with Toll-like receptors showed a high affinity and low constant of dissociation, which could lead to an innate-type immune response that helps to eliminate the parasite. In conclusion, the consensus sequence proposed for enolase is capable of providing an ideal immune response; however, the experimental evaluation of this enolase consensus and their chimeric peptides should be a high priority to develop a vaccine against Chagas disease.
目前尚无针对由该寄生虫引起的美洲锥虫病的疫苗。这是由于在该寄生虫的六个离散型（DTUs）中观察到基因组变异。这项工作旨在提出来自不同菌株的烯醇化酶蛋白的共有序列，并主要在生物信息学水平评估其免疫原性。从专业数据库中，对15个烯醇化酶基因序列进行比对以获得共有序列，对该序列进行建模，然后通过不同的生物信息学程序进行评估和验证，以了解其免疫原潜力。最后，设计了具有最具代表性表位的嵌合肽。结果显示，该共有序列具有较高的免疫原潜力，有六个主要组织相容性复合体I类（MHC-I）表位和七个MHC-II类表位，所有这些表位都高度代表了美洲大陆菌株中存在的烯醇化酶，还有五个B细胞表位。关于计算建模，与Toll样受体的分子对接显示出高亲和力和低解离常数，这可能导致有助于消除寄生虫的先天性免疫反应。总之，所提出的烯醇化酶共有序列能够提供理想的免疫反应；然而，对这种烯醇化酶共有序列及其嵌合肽进行实验评估应成为开发恰加斯病疫苗的高度优先事项。
