de Jong Feiko J M, Brinkman Paul, Wingelaar Thijs T, van Ooij Pieter-Jan A M, van Hulst Rob A
Royal Netherlands Navy Diving and Submarine Medical Centre, 1780 CA Den Helder, The Netherlands.
Department of Anesthesiology, Amsterdam UMC, Location AMC, 1100 DD Amsterdam, The Netherlands.
Metabolites. 2022 May 23;12(5):470. doi: 10.3390/metabo12050470.
Diving or hyperbaric oxygen therapy with increased partial pressures of oxygen (pO) can have adverse effects such as central nervous system oxygen toxicity or pulmonary oxygen toxicity (POT). Prevention of POT has been a topic of interest for several decades. One of the most promising techniques to determine early signs of POT is the analysis of volatile organic compounds (VOCs) in exhaled breath. We reanalyzed the data of five studies to compose a library of potential exhaled markers for the early detection of POT. GC-MS data from five hyperbaric hyperoxic studies were collected. Wilcoxon signed-rank tests were used to compare baseline- and postexposure measurements; all ion fragments that significantly varied were compared by similarity using the National Institute of Standards and Technology (NIST) library. All identified molecules were cross-referenced with open-source databases and other scientific publications on VOCs to exclude compounds that occurred as a result of contamination, and to identify the compounds most likely to occur due to hyperbaric hyperoxic exposure. After identification and removal of contaminants, 29 compounds were included in the library. This library of hyperbaric hyperoxic-related VOCs can help to advance the development of an early noninvasive marker of POT. It enables validation by others who use more targeted MS-related techniques, instead of full-scale GC-MS, for their exhaled VOC research.
潜水或采用增加氧分压（pO）的高压氧疗法可能会产生诸如中枢神经系统氧中毒或肺氧中毒（POT）等不良反应。几十年来，预防POT一直是人们关注的话题。确定POT早期迹象最有前景的技术之一是分析呼出气中的挥发性有机化合物（VOCs）。我们重新分析了五项研究的数据，以构建一个用于早期检测POT的潜在呼出标记物库。收集了五项高压高氧研究的气相色谱-质谱（GC-MS）数据。采用Wilcoxon符号秩检验比较基线测量值和暴露后测量值；使用美国国家标准与技术研究院（NIST）库通过相似性比较所有显著变化的离子碎片。将所有鉴定出的分子与开源数据库以及其他关于VOCs的科学出版物进行交叉引用，以排除因污染而出现的化合物，并确定最有可能因高压高氧暴露而出现的化合物。在鉴定并去除污染物后，该库中包含了29种化合物。这个与高压高氧相关的VOCs库有助于推动POT早期非侵入性标记物的开发。它能够让其他在呼出VOC研究中使用更具针对性的质谱相关技术而非全规模GC-MS的研究人员进行验证。
