de Jong Feiko J M, Lilien Thijs A, Fenn Dominic W, Wingelaar Thijs T, van Ooij Pieter-Jan A M, Maitland-van der Zee Anke H, Hollmann Markus W, van Hulst Rob A, Brinkman Paul
Royal Netherlands Navy Diving and Submarine Medical Centre, 1780 CA Den Helder, The Netherlands.
Department of Anesthesiology, Amsterdam UMC, Location AMC, 1100 DD Amsterdam, The Netherlands.
Metabolites. 2024 May 13;14(5):281. doi: 10.3390/metabo14050281.
Volatile organic compounds (VOCs) might be associated with pulmonary oxygen toxicity (POT). This pilot study aims to identify VOCs linked to oxidative stress employing an in vitro model of alveolar basal epithelial cells exposed to hyperbaric and hyperoxic conditions. In addition, the feasibility of this in vitro model for POT biomarker research was evaluated. The hyperbaric exposure protocol, similar to the U.S. Navy Treatment Table 6, was conducted on human alveolar basal epithelial cells, and the headspace VOCs were analyzed using gas chromatography-mass spectrometry. Three compounds (nonane [ = 0.005], octanal [ = 0.009], and decane [ = 0.018]), of which nonane and decane were also identified in a previous in vivo study with similar hyperbaric exposure, varied significantly between the intervention group which was exposed to 100% oxygen and the control group which was exposed to compressed air. VOC signal intensities were lower in the intervention group, but cellular stress markers (IL8 and LDH) confirmed increased stress and injury in the intervention group. Despite the observed reductions in compound expression, the model holds promise for POT biomarker exploration, emphasizing the need for further investigation into the complex relationship between VOCs and oxidative stress.
挥发性有机化合物（VOCs）可能与肺氧中毒（POT）有关。这项初步研究旨在利用暴露于高压高氧条件下的肺泡基底上皮细胞体外模型，确定与氧化应激相关的VOCs。此外，还评估了该体外模型用于POT生物标志物研究的可行性。对人肺泡基底上皮细胞进行了类似于美国海军治疗表6的高压暴露方案，并使用气相色谱-质谱法分析顶空VOCs。三种化合物（壬烷[=0.005]、辛醛[=0.009]和癸烷[=0.018]），其中壬烷和癸烷也在先前一项具有类似高压暴露的体内研究中被鉴定出，在暴露于100%氧气的干预组和暴露于压缩空气的对照组之间有显著差异。干预组的VOC信号强度较低，但细胞应激标志物（IL8和LDH）证实干预组的应激和损伤增加。尽管观察到化合物表达降低，但该模型有望用于POT生物标志物探索，强调需要进一步研究VOCs与氧化应激之间的复杂关系。
