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超高效液相色谱-二次离子阱-飞行时间质谱联用技术用于鉴定云南白药活血化瘀作用的代谢靶标

UPLC-G2Si-HDMS Untargeted Metabolomics for Identification of Yunnan Baiyao's Metabolic Target in Promoting Blood Circulation and Removing Blood Stasis.

机构信息

National Engineering Laboratory for the Development of Southwestern Endangered Medicinal Materials, Guangxi Botanical Garden of Medicinal Plant, Nanning 530000, China.

National Chinmedomics Research Center, National TCM Key Laboratory of Serum Pharmacochemistry, Metabolomics Laboratory, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.

出版信息

Molecules. 2022 May 17;27(10):3208. doi: 10.3390/molecules27103208.

DOI:10.3390/molecules27103208
PMID:35630682
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9143197/
Abstract

Yunnan Baiyao is a famous Chinese patent medicine in Yunnan Province. However, its mechanism for promoting blood circulation and removing blood stasis is not fully explained. Our study used metabonomics technology to reveal the regulatory effect of Yunnan Baiyao on small molecular metabolites in promoting blood circulation and removing blood stasis, and exploring the related urine biomarkers. The coagulation function, blood rheology, and pathological results demonstrated that after Yunnan Baiyao treatment, the pathological indexes in rats with epinephrine hydrochloride-induced blood stasis syndrome improved and returned to normal levels. This is the basis for the effectiveness of Yunnan Baiyao. UPLC-G2Si-HDMS was used in combination with multivariate statistical analysis to conduct metabonomic analysis of urine samples. Finally, using mass spectrometry technology, 28 urine biomarkers were identified, clarifying the relevant metabolic pathways that play a vital role in the Yunnan Baiyao treatment. These were used as the target for Yunnan Baiyao to promote blood circulation and remove blood stasis. This study showed that metabolomics strategies provide opportunities and conditions for a deep and systematic understanding of the mechanism of action of prescriptions.

摘要

云南白药是云南省的一种著名的中成药。然而，其活血化瘀的作用机制尚未完全阐明。本研究采用代谢组学技术揭示了云南白药在活血化瘀方面对小分子代谢物的调节作用，并探索了相关的尿生物标志物。凝血功能、血液流变学和病理结果表明，云南白药治疗后，盐酸肾上腺素致血瘀证大鼠的病理指标得到改善并恢复正常水平。这是云南白药有效性的基础。采用 UPLC-G2Si-HDMS 结合多元统计分析方法对尿液样本进行代谢组学分析。最后，利用质谱技术鉴定了 28 个尿生物标志物，阐明了在云南白药治疗中起重要作用的相关代谢途径。这些被用作云南白药活血化瘀的作用靶点。本研究表明，代谢组学策略为深入系统地了解方剂的作用机制提供了机会和条件。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/50183a913c37/molecules-27-03208-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/6e6fa7fbf199/molecules-27-03208-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/ef8cf1f012cc/molecules-27-03208-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/a61a0cffb954/molecules-27-03208-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/386f8b7a694b/molecules-27-03208-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/7a080109617e/molecules-27-03208-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/1ef5c83c0986/molecules-27-03208-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/34bd4b7d3754/molecules-27-03208-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/82535083ec2d/molecules-27-03208-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/233c9edeae86/molecules-27-03208-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/50183a913c37/molecules-27-03208-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/6e6fa7fbf199/molecules-27-03208-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/ef8cf1f012cc/molecules-27-03208-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/a61a0cffb954/molecules-27-03208-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/386f8b7a694b/molecules-27-03208-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/7a080109617e/molecules-27-03208-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/1ef5c83c0986/molecules-27-03208-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/34bd4b7d3754/molecules-27-03208-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/82535083ec2d/molecules-27-03208-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/233c9edeae86/molecules-27-03208-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0603/9143197/50183a913c37/molecules-27-03208-g010.jpg

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