Tolón R M, Obispo T, Alava I, Navarro J L, García-Frade L J
Rev Esp Fisiol. 1986 Dec;42(4):535-41.
This work compares two distinct methods, column and batch, for the purification of fibronectin, from different plasma fractions, using gelatin and one of its derivates (Hemoce, Behring). The yields of both techniques at quantitative as well as qualitative levels (levels of immunologically active fibronectin), are evaluated. The data indicate that better conservation of immunological immunological characteristics is obtained with the use of gelatin derivatives (Hemoce). The plasma fraction does not have significant influence on the process yields.
这项工作比较了两种不同的方法，即柱法和批量法，用于从不同血浆组分中使用明胶及其一种衍生物（赫莫塞，贝林）纯化纤连蛋白。评估了两种技术在定量以及定性水平（免疫活性纤连蛋白水平）上的产量。数据表明，使用明胶衍生物（赫莫塞）能更好地保留免疫特性。血浆组分对工艺产量没有显著影响。
