Borsi L, Castellani P, Balza E, Siri A, Pellecchia C, De Scalzi F, Zardi L
Anal Biochem. 1986 Jun;155(2):335-45. doi: 10.1016/0003-2697(86)90443-4.
Human plasma fibronectin is composed of at least seven distinct domains, with affinities for different macromolecules and cell surfaces. Here we describe in detail a simple high-yield procedure for the purification of large amounts of fibronectin domains. This involves thermolysin digestion of the fibronectin molecule followed by the purification of the domain using mainly hydroxyapatite chromatography columns. This procedure represents a great simplification over those previously reported.
人血浆纤连蛋白由至少七个不同结构域组成，对不同的大分子和细胞表面具有亲和力。在此，我们详细描述一种简单的高产方法，用于大量纯化纤连蛋白结构域。该方法包括用嗜热菌蛋白酶消化纤连蛋白分子，然后主要使用羟基磷灰石柱对该结构域进行纯化。此方法相较于先前报道的方法有了极大简化。
