Sárközi Rita, Makrai László, Fodor László
Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, P.O. Box 22, H-1581 Budapest, Hungary.
Pathogens. 2022 Apr 24;11(5):505. doi: 10.3390/pathogens11050505.
is a major pathogen of swine, which can cause severe pleuropneumonia in pigs, but sometimes the disease can be generalized. Diseases caused by are frequent all over the world, resulting in high losses among domestic pigs. However, our knowledge on the occurrence of in wild boars and feral pigs is limited. We aimed to examine the carriage of by hunted wild boars. The presence of was examined in tonsils of 68 hunted wild boars collected at a game processing unit. An in-house designed species-specific PCR test was used to detect the gene of Apx IV toxin, and the samples were inoculated on a modified selective agar. was detected in 10 animals (14.7%) by PCR and one serotype 12 strain was isolated. The antibiotic resistance pattern of the strain resembled field strains that were isolated from farmed pigs in Hungary. This is the first case for the detection of not only using PCR or ELISA, but also its isolation, identification, and serotyping.
是猪的主要病原体，可导致猪严重的胸膜肺炎，但有时疾病会全身性发作。由其引起的疾病在世界各地频发，给家猪造成了巨大损失。然而，我们对野猪和野猪中该病原体的发生情况了解有限。我们旨在检测被猎杀野猪携带该病原体的情况。在一个野味加工单位收集的68头被猎杀野猪的扁桃体中检测该病原体的存在。使用内部设计的种特异性PCR试验检测Apx IV毒素基因，并将样本接种在改良的选择性琼脂上。通过PCR在10只动物（14.7%）中检测到该病原体，并分离出一株血清型12菌株。该菌株的抗生素耐药模式与从匈牙利养殖猪中分离出的田间菌株相似。这是不仅使用PCR或ELISA检测该病原体，还对其进行分离、鉴定和血清分型的首例。
