用于检测胸膜肺炎放线杆菌Apx毒素抗体的间接酶联免疫吸附测定（ELISA）的评估

Evaluation of an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the Apx toxins of Actinobacillus pleuropneumoniae.

作者信息

Nielsen R, van den Bosch J F, Plambeck T, Sørensen V, Nielsen J P

机构信息

Danish Veterinary Laboratory, Copenhagen, Denmark.

出版信息

Vet Microbiol. 2000 Jan;71(1-2):81-7. doi: 10.1016/s0378-1135(99)00157-1.

DOI:10.1016/s0378-1135(99)00157-1

PMID:10665536

Abstract

The reference strains of the 12 serotypes of Actinobacillus pleuropneumoniae express one or two of three different RTX exotoxins designated Apx I, Apx II and Apx III. The toxins are important virulence factors. In the present study, ELISAs with purified Apx I, Apx II and Apx III, respectively, as antigen were evaluated as candidates for serological diagnosis of Actinobacillus pleuropneumoniae infection in pigs. The pigs were inoculated with biotype 1, serotypes 1-12, and biotype 2, serotype 14, respectively. A strong humoral antibody response was seen to all the three antigens in most pigs irrespective of the serotype used for inoculation. However, titers to the exotoxins secreted by the serotype used for inoculation were generally highest. The results show that toxin proteins of Actinobacillus pleuropneumoniae are antigenically related and that a correlation between serotype and secretion of exotoxin is not revealed serologically in the ELISA test.

摘要

胸膜肺炎放线杆菌12种血清型的参考菌株表达三种不同RTX外毒素（分别命名为Apx I、Apx II和Apx III）中的一种或两种。这些毒素是重要的毒力因子。在本研究中，分别以纯化的Apx I、Apx II和Apx III作为抗原的酶联免疫吸附测定（ELISA）被评估为猪胸膜肺炎放线杆菌感染血清学诊断的候选方法。分别用生物型1、血清型1 - 12以及生物型2、血清型14接种猪。无论接种所用的血清型如何，大多数猪对所有三种抗原均呈现强烈的体液抗体反应。然而，针对接种所用血清型分泌的外毒素的效价通常最高。结果表明，胸膜肺炎放线杆菌的毒素蛋白具有抗原相关性，并且在ELISA试验中血清学上未揭示血清型与外毒素分泌之间的相关性。