Chen Yandi, Yang Bina, Cheng Lei, Xu Hockin H K, Li Hao, Huang Yuyao, Zhang Qiong, Zhou Xuedong, Liang Jingou, Zou Jing
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Department of Pediatric Dentistry, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Pathogens. 2022 May 14;11(5):578. doi: 10.3390/pathogens11050578.
The objective of this study was to develop novel modified giomers by incorporating the antibacterial quaternary ammonium monomers (QAMs), dimethylaminododecyl methacrylate (DMADDM) or dimethylaminohexadecyl methacrylate (DMAHDM) into a commercial giomer. The material performances including mechanical properties, surface characteristics, color data, cytotoxicity and fluoride release of the novel giomers were evaluated. Antibacterial activity against severe early childhood caries (S-ECC) saliva-derived biofilms was assessed by lactic acid production measurement, MTT assay, biofilm staining and 16S rRNA sequencing. A rat model was developed and the anti-caries effect was investigated by micro-CT scanning and modified Keyes' scoring. The results showed that the material properties of the QAMs groups were comparable to those of the control group. The novel giomers significantly inhibited lactic acid production and biofilm viability of S-ECC saliva-derived biofilms. Furthermore, caries-related genera such as and reduced in QAMs groups, which showed their potential to change the microbial compositions. In the rat model, lesion depth, mineral loss and scoring of the QAMs groups were significantly reduced, without side effects on oral tissues. In conclusion, the novel giomers incorporated with antibacterial QAMs could inhibit the cariogenic biofilms and help prevent secondary caries, with great potential for future application in restorative treatment.
本研究的目的是通过将抗菌季铵单体（QAMs）、甲基丙烯酸二甲氨基十二烷基酯（DMADDM）或甲基丙烯酸二甲氨基十六烷基酯（DMAHDM）掺入商用聚硅氧烷中，开发新型改性聚硅氧烷。对新型聚硅氧烷的材料性能进行了评估，包括机械性能、表面特性、颜色数据、细胞毒性和氟释放。通过乳酸产量测定、MTT法、生物膜染色和16S rRNA测序评估了对重度幼儿龋（S-ECC）唾液来源生物膜的抗菌活性。建立了大鼠模型，并通过显微CT扫描和改良的凯斯评分法研究了防龋效果。结果表明，QAMs组的材料性能与对照组相当。新型聚硅氧烷显著抑制了S-ECC唾液来源生物膜的乳酸产生和生物膜活力。此外，QAMs组中与龋齿相关的属如 和 减少，这表明它们有改变微生物组成的潜力。在大鼠模型中，QAMs组的病变深度、矿物质流失和评分显著降低，对口腔组织无副作用。总之，掺入抗菌QAMs的新型聚硅氧烷可以抑制致龋生物膜并有助于预防继发龋，在修复治疗中具有巨大的未来应用潜力。
