Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.
Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University, Beijing 100191, China.
Nutrients. 2022 May 13;14(10):2051. doi: 10.3390/nu14102051.
Aging-related muscle loss is a hallmark of aging and is the cause of some negative outcomes. An optimized diet and supplements have a positive effect in slowing down the process of muscle loss. This study was designed to evaluate the beneficial effects of walnut oligopeptides (WOPs) on aging-related muscle loss and explore the possible underlying mechanism in Senescence-Accelerated Mouse Prone 8 (SAMP8) Mice. SAMP8 mice were randomly divided into four groups ( = 15/group), including one group which was the SAMP8 age control group and three groups those were WOP intervention groups. Meanwhile, Senescence Accelerated Resistant Mouse 1 (SAMR1) mice ( = 12), which had normal senescence rates, were used as model controls. During the six-month intervention period, the age control and normal control groups were given sterilized water, while the three WOP intervention groups were given WOP solution with low (110 mg/kg·bw), medium (220 mg/kg·bw) and high concentrations (440 mg/kg·bw), respectively. The results showed that WOPs could significantly increase muscle mass and improve physical performance (wire hang and catwalk behavioral tests) in aging mice. Moreover, WOPs could significantly reduce the levels of IL-1β, IL-6 and TNF-α in serum and gastrocnemius tissues and increase the mitochondrial DNA content, as well as the expression levels of AMPK, PGC-1α, NRF-1 and TFAM in the gastrocnemius muscle of aging mice, which was speculated to be the specific mechanism related to mitochondrial function improvement and inflammation reduction. These results indicate that WOPs can improve aging-related muscle loss, in term of both muscle mass and physical performance, and WOP supplements seems to be potentially effective in elderly individuals.
衰老是肌肉损失的一个标志，也是一些负面结果的原因。优化饮食和补充剂对减缓肌肉损失的过程有积极的影响。本研究旨在评估核桃寡肽（WOPs）对与衰老相关的肌肉损失的有益作用，并探讨其在快速老化小鼠 8 号（SAMP8）小鼠中的可能潜在机制。将 SAMP8 小鼠随机分为四组（每组 15 只），包括一组 SAMP8 年龄对照组和三组 WOP 干预组。同时，使用衰老加速抵抗小鼠 1 号（SAMR1）小鼠（正常衰老率）作为模型对照。在六个月的干预期间，年龄对照组和正常对照组给予无菌水，而三个 WOP 干预组分别给予低（110mg/kg·bw）、中（220mg/kg·bw）和高浓度（440mg/kg·bw）的 WOP 溶液。结果表明，WOPs 可显著增加衰老小鼠的肌肉质量并改善其身体机能（悬线和猫道行为测试）。此外，WOPs 可显著降低血清和比目鱼肌组织中 IL-1β、IL-6 和 TNF-α的水平，增加线粒体 DNA 含量，以及衰老小鼠比目鱼肌中 AMPK、PGC-1α、NRF-1 和 TFAM 的表达水平，这被推测是与改善线粒体功能和减轻炎症相关的特定机制。这些结果表明，WOPs 可改善与衰老相关的肌肉损失，无论是肌肉质量还是身体机能，并且 WOP 补充剂似乎对老年人具有潜在的有效性。
