BK21 FOUR Program, College of Veterinary Medicine, Chungnam National University, 99 Daehak-ro, Daejeon 34131, Korea.
BK21 FOUR Program, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Korea.
Nutrients. 2022 May 15;14(10):2067. doi: 10.3390/nu14102067.
The inner shell of the chestnut (Castanea crenata) contains various polyphenols, which exert beneficial biological effects. Hence, we assessed the anti-inflammatory efficacy of a chestnut inner shell extract (CIE) in ovalbumin (OVA)-induced allergic asthma. We intraperitoneally injected 20 μg of OVA with 2 mg of aluminum hydroxide on days 0 and 14. On test days 21, 22, and 23, the mice were treated with aerosolized 1% () OVA in saline. CIE was administered orally at 100 and 300 mg/kg on days 18-23. CIE significantly reduced inflammatory cytokines and cells and immunoglobulin-E increased by OVA. Anti-inflammatory efficacy was revealed by reduction of inflammatory cell migration and mucus secretion in lung tissue. Further, CIE suppressed the OVA-induced nuclear factor kappa B (NF-κB) phosphorylation. Accordingly, the expression of cyclooxygenase (COX-2), inducible nitric oxide synthase (iNOS), and matrix metalloproteinase-9 (MMP-9) were decreased sequentially in lung tissues. CIE alleviated OVA-induced airway inflammation by restraining phosphorylation of NF-κB and the sequentially reduced expression of iNOS, COX-2, leading to reduced MMP-9 expression. These results indicate that CIE has potential as a candidate for alleviating asthma.
板栗（Castanea crenata）内壳含有多种多酚，具有有益的生物学作用。因此，我们评估了板栗内壳提取物（CIE）在卵清蛋白（OVA）诱导的过敏性哮喘中的抗炎功效。我们在第 0 天和第 14 天经腹腔注射 20μg OVA 和 2mg 氢氧化铝。在测试第 21、22 和 23 天，用生理盐水雾化 1%（）OVA 对小鼠进行处理。在第 18-23 天，CIE 以 100 和 300mg/kg 的剂量口服给药。CIE 显著降低了由 OVA 引起的炎性细胞因子和细胞以及免疫球蛋白-E 的增加。通过减少肺组织中炎症细胞迁移和粘液分泌来显示抗炎功效。此外，CIE 抑制了 OVA 诱导的核因子 kappa B（NF-κB）磷酸化。相应地，肺组织中依次降低了环氧合酶（COX-2）、诱导型一氧化氮合酶（iNOS）和基质金属蛋白酶-9（MMP-9）的表达。CIE 通过抑制 NF-κB 的磷酸化和随之降低的 iNOS、COX-2 表达，从而降低 MMP-9 表达，减轻 OVA 诱导的气道炎症。这些结果表明 CIE 具有缓解哮喘的潜力。
