Jia Hongyu, Chen Junjie, Zhang Lin, Zhang Lingling
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, College of Forestry, Central South University of Forestry and Technology, Changsha 410000, China.
CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430074, China.
Plants (Basel). 2022 May 16;11(10):1315. doi: 10.3390/plants11101315.
Tung tree () is an industrially important oil-bearing woody plant of the Euphorbiaceae family. Functional studies of tung tree at the molecular level are limited by the lack of an efficient transgenic system. The -mediated hairy root generation system is an alternative to typical plant transformation systems. However, its application in many plants has been blocked due to the inability of existing methods to induce hairy roots. Thus, it is critical to build a method suitable for the hairy induction of the specific plant of interest. In this study, a modified method for tung tree was developed, and it is the first report that hairy roots could be effectively induced in the stem of tung tree. With the method, an average of 10.7 hairy roots per seedling were generated in tung tree, approximately 67% of seedlings produced transgenic hairy roots and approximately 13.96% of the hairy roots of these seedlings were transgenic. This modified method is also suitable for the hairy root induction of two other oil-bearing plants of the Euphorbiaceae family, and . This modified method will accelerate functional studies of tung tree at the molecular level and also shed light on plants lacking a transgenic system.
油桐（Vernicia fordii）是大戟科一种具有重要工业价值的含油木本植物。由于缺乏高效的转基因系统，油桐在分子水平上的功能研究受到限制。发根农杆菌介导的毛状根生成系统是典型植物转化系统的一种替代方法。然而，由于现有方法无法诱导毛状根，其在许多植物中的应用受到阻碍。因此，建立一种适合特定目标植物毛状根诱导的方法至关重要。在本研究中，开发了一种改良的油桐方法，这是首次报道能够在油桐茎中有效诱导毛状根。采用该方法，油桐每株幼苗平均产生10.7条毛状根，约67%的幼苗产生转基因毛状根，这些幼苗中约13.96%的毛状根为转基因根。这种改良方法也适用于大戟科另外两种含油植物，麻疯树（Jatropha curcas）和乌桕（Sapium sebiferum）的毛状根诱导。这种改良方法将加速油桐在分子水平上的功能研究，也为缺乏转基因系统的植物提供启示。
