Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, 410004, Hunan, China; Key Lab of Non-wood Forest Products of State Forestry Administration, College of Forestry, Central South University of Forestry and Technology, Changsha, 410004, Hunan, China.
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, 410004, Hunan, China; Key Lab of Non-wood Forest Products of State Forestry Administration, College of Forestry, Central South University of Forestry and Technology, Changsha, 410004, Hunan, China.
Phytochemistry. 2021 May;185:112686. doi: 10.1016/j.phytochem.2021.112686. Epub 2021 Feb 12.
Leucine-rich repeat receptor-like protein kinases (LRR-RLKs) are vital for plant growth and development, signal transduction, immunity, and play diverse roles in plant defense responses. However, the LRR-RLK genes have not been systematically studied in Vernicia fordii (tung tree), especially its response to Fusarium wilt. Here, we carried out an integrative analysis of LRR-RLKs among five Euphorbiaceae species: Hevea brasiliensis (rubber tree), Manihot esculenta (cassava), Jatropha curcas (physic nut), Ricinus communis (castor bean), and V. fordii, which contained 223, 311, 186, 138, and 167 LRR-RLKs, respectively. Maximum-likelihood tree was estimated using LRR-RLKs of Arabidopsis thaliana as a template, and they allowed us to divide Euphorbiaceae LRR-RLKs into 22 groups. There are 126 segmental and 30 tandem duplications in these Euphorbiaceae genomes by synteny analysis. The tissue-specific expression patterns revealed that V. fordii LRR-RLKs (VfLRR-RLKs) were differentially expressed in various tissues, and some of them exhibited specific expression in meristems tissues, which suggested their potential functions during organ formation and cell fate specification. Two VfLRR-RLK pairs (Vf01G2125 and Vf03G1740, Vf06G2687 and Vf10G1659), which generated by tandem duplication events, were associated with possible resistance to Fusarium wilt infection. The qRT-PCR confirmed these four VfLRR-RLKs contained opposite expression profiles during pathogen infection in V. fordii and V. montana. Taken together, our data systematically analyzed the LRR-RLK family in Euphorbiaceae genomes for the first time. We highlight the putative roles of VfLRR-RLKs in response to Fusarium wilt infection, and VfLRR-RLKs may be further applied in marker-assisted breeding to control Fusarium wilt in V. fordii.
富含亮氨酸重复受体样蛋白激酶（LRR-RLKs）在植物生长发育、信号转导、免疫等方面发挥着重要作用，并在植物防御反应中发挥着多样化的作用。然而，在油桐（桐树）中，LRR-RLK 基因尚未得到系统研究，特别是其对枯萎病的反应。在这里，我们对 5 种大戟科植物中的 LRR-RLK 进行了综合分析：巴西橡胶树（橡胶树）、木薯（木薯）、麻疯树（麻疯树）、蓖麻（蓖麻）和油桐（油桐），分别包含 223、311、186、138 和 167 个 LRR-RLK。使用拟南芥的 LRR-RLK 作为模板对最大似然树进行估计，这使我们能够将大戟科 LRR-RLK 分为 22 组。通过共线性分析，这些大戟科基因组中存在 126 个片段和 30 个串联重复。组织特异性表达模式表明，油桐 LRR-RLK（VfLRR-RLKs）在不同组织中差异表达，其中一些在分生组织组织中特异性表达，这表明它们在器官形成和细胞命运特化过程中具有潜在的功能。两个 VfLRR-RLK 对（Vf01G2125 和 Vf03G1740、Vf06G2687 和 Vf10G1659）通过串联重复事件产生，与可能对枯萎病感染的抗性有关。qRT-PCR 证实，这四个 VfLRR-RLK 在油桐和油桐感染病原体时的表达模式相反。总之，我们的数据首次系统地分析了大戟科基因组中的 LRR-RLK 家族。我们强调了 VfLRR-RLK 在应对枯萎病感染中的可能作用，并且 VfLRR-RLK 可能进一步应用于标记辅助育种，以控制油桐中的枯萎病。
