Department of Virology, University of KwaZulu Natal and National Health Laboratory Service, Durban 4000, South Africa.
Medical Research Council-University of Glasgow Centre for Virus Research, Bearsden, Glasgow G61 1QH, UK.
Viruses. 2022 Apr 21;14(5):855. doi: 10.3390/v14050855.
Human cytomegalovirus (HCMV) can cause significant end-organ diseases such as pneumonia in HIV-exposed infants. Complex viral factors may influence pathogenesis including: a large genome with a sizeable coding capacity, numerous gene regions of hypervariability, multiple-strain infections, and tissue compartmentalization of strains. We used a whole genome sequencing approach to assess the complexity of infection by comparing high-throughput sequencing data obtained from respiratory and blood specimens of HIV-exposed infants with severe HCMV pneumonia with those of lung transplant recipients and patients with hematological disorders. There were significantly more specimens from HIV-exposed infants showing multiple HCMV strain infection. Some genotypes, such as UL73 G4B and UL74 G4, were significantly more prevalent in HIV-exposed infants with severe HCMV pneumonia. Some genotypes were predominant in the respiratory specimens of several patients. However, the predominance was not statistically significant, precluding firm conclusions on anatomical compartmentalization in the lung.
人巨细胞病毒（HCMV）可导致明显的终末器官疾病，如 HIV 暴露婴儿的肺炎。复杂的病毒因素可能影响发病机制，包括：具有相当编码能力的大型基因组、多个高度变异的基因区域、多种毒株感染和毒株的组织区隔化。我们使用全基因组测序方法通过比较从 HIV 暴露婴儿重症 HCMV 肺炎的呼吸道和血液标本中获得的高通量测序数据与肺移植受者和血液系统疾病患者的数据，来评估感染的复杂性。显示多重 HCMV 株感染的 HIV 暴露婴儿的标本明显更多。一些基因型，如 UL73 G4B 和 UL74 G4，在 HIV 暴露婴儿重症 HCMV 肺炎中更为常见。一些基因型在数名患者的呼吸道标本中占优势。然而，这种优势没有统计学意义，因此无法就肺部的解剖分区得出确定的结论。
