在喂以 Teklad 2920x 啮齿动物饲料而非 LabDiet 5001 的情况下，C57BL/6J 小鼠的酒精前负荷发展（而非表达）被消除。
The development, but not expression, of alcohol front-loading in C57BL/6J mice maintained on LabDiet 5001 is abolished by maintenance on Teklad 2920x rodent diet.
机构信息
Department of Neurosciences, University of New Mexico, Albuquerque, New Mexico, USA.
出版信息
Alcohol Clin Exp Res. 2022 Jul;46(7):1321-1330. doi: 10.1111/acer.14876. Epub 2022 Jun 8.
BACKGROUND
Excessive alcohol (ethanol) consumption, such as binge drinking, is extremely commonplace and represents a major health concern. Through modeling excessive drinking in rodents, we are beginning to uncover the neurobiological and neurobehavioral causes and consequences of this pattern of ethanol intake. One important factor for modeling binge drinking in mice is that they reliably drink to blood ethanol concentrations (BECs) of 80 mg/dl or higher. Drinking-in-the-dark (DID) is a commonly used mouse model of binge drinking, and we have shown that this method reliably results in robust ethanol front-loading and binge-level BECs in C57BL/6J (B6) mice and other ethanol-preferring mouse strains/lines. However, establishing the DID model in a new vivarium space forced us to consider the use of rodent diet formulations that we had not previously used.
METHODS
The current set of experiments were designed to investigate the role of two standard rodent diet formulations on binge drinking and the development of ethanol front-loading using DID.
RESULTS
We found that BECs in animals maintained on LabDiet 5001 (LD01) were double those found in mice maintained on Teklad 2920x (TL20). Interestingly, this effect was paralleled by differences in the degree of front-loading, such that LD01-fed mice consumed approximately twice as much ethanol in the first 15 min of the 2-h DID sessions as the TL20-fed mice. Surprisingly, however, mice that developed front-loading during maintenance on the LD01 diet continued to display front-loading behavior after being switched to the TL20 diet.
CONCLUSIONS
These data emphasize the importance of choosing and reporting diet formulations when conducting voluntary drinking studies and support the need for further investigation into the mechanisms behind diet-induced differences in binge drinking, particularly front-loading.
背景
过度饮酒（乙醇），如狂饮，极其普遍，是一个主要的健康关注点。通过在啮齿动物中模拟过度饮酒，我们开始揭示这种乙醇摄入模式的神经生物学和神经行为原因和后果。在小鼠中模拟狂饮的一个重要因素是，它们会可靠地将血液乙醇浓度（BEC）喝到 80mg/dl 或更高。暗饮（DID）是一种常用的小鼠狂饮模型，我们已经表明，这种方法可靠地导致 C57BL/6J（B6）小鼠和其他乙醇偏好的小鼠品系/品系中强烈的乙醇前加载和狂饮水平的 BEC。然而，在新的动物饲养室空间中建立 DID 模型迫使我们考虑使用以前未使用过的啮齿动物饮食配方。
方法
本实验集旨在研究两种标准啮齿动物饮食配方对 DID 下的狂饮和乙醇前加载发展的作用。
结果
我们发现，维持在 LabDiet 5001（LD01）上的动物的 BEC 是维持在 Teklad 2920x（TL20）上的动物的两倍。有趣的是，这种效果与前加载程度的差异相平行，即 LD01 喂养的小鼠在 2 小时 DID 期间的前 15 分钟内消耗的乙醇量大约是 TL20 喂养的小鼠的两倍。然而，令人惊讶的是，在 LD01 饮食维持期间发展出前加载行为的小鼠在切换到 TL20 饮食后继续表现出前加载行为。
结论
这些数据强调了在进行自愿饮酒研究时选择和报告饮食配方的重要性，并支持进一步研究饮食引起的狂饮差异背后的机制，特别是前加载。
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