Department of Internal Medicine, Nepalgunj Medical College and Teaching Hospital, Nepalgunj, Banke, Nepal.
Department of Microbiology, Nepalgunj Medical College and Teaching Hospital, Nepalgunj, Banke, Nepal.
JNMA J Nepal Med Assoc. 2022 Mar 11;60(247):250-253. doi: 10.31729/jnma.7329.
Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis mostly affecting the lungs. Due to the low sensitivity of conventional microscopy and time-consuming culture method, Nucleic acid Amplification Assay Technique is preferred because of its rapidity and sensitivity. This test also helps in finding drug resistance to Rifampicin and also curtails the transmission of disease. The study is aimed to find the prevalence of GeneXpert confirmed cases among suspected cases of tuberculosis in a tertiary care centre.
A descriptive cross-sectional study in 104 patients was conducted in a tertiary care centre from 30th Dec 2021 to 3rd Feb 2022. Ethical clearance was taken from the Institutional Review Committee (Reference number: 464/078/079). Sputum samples were collected from patients and were processed for GeneXpert under biological safety standards. GeneXpert Mycobacterium tuberculosis/rifampicin assay, sample processing, deoxyribonucleic acid extraction, and deoxyribonucleic acid amplification occurred in a fully automated cartridge-based real-time Polymerase chain reaction. A convenience sampling method was done. Collected data were coded as per variables and entered in Statistical Package for the Social Sciences version 25. Point estimate at 95% Confidence Interval was calculated along with frequency and percentage for binary data.
In all 104 patients, GeneXpert detected 10 (9.62%) (3.94-15.26 at 95% Confidence Interval) positive tuberculosis cases. Out of total positive cases, there were 6 (60%) males and 4 (40%) females and there was 1 (10%) rifampicin-resistant case.
The prevalence of pulmonary tuberculosis among presumptive cases in our study was found to be similar to reported literature.
multidrug-resistant; nucleic acid amplification test; pulmonary tuberculosis.
结核病是一种由结核分枝杆菌引起的传染病，主要影响肺部。由于传统显微镜检查的敏感性低和培养方法耗时，核酸扩增检测技术因其快速和敏感而受到青睐。该检测还有助于发现利福平耐药性，并减少疾病的传播。本研究旨在发现三级保健中心疑似结核病患者中 GeneXpert 确诊病例的流行情况。
在 2021 年 12 月 30 日至 2022 年 2 月 3 日期间，在一家三级保健中心对 104 例患者进行了描述性横断面研究。本研究已获得机构审查委员会的伦理批准（参考号：464/078/079）。按照生物安全标准从患者中采集痰液样本，并进行 GeneXpert 检测。GeneXpert 结核分枝杆菌/利福平检测、样本处理、脱氧核糖核酸提取和脱氧核糖核酸扩增均在基于全自动盒式实时聚合酶链反应的仪器中进行。采用方便抽样法。收集的数据根据变量进行编码，并输入到统计软件包 25 版中。计算了 95%置信区间的点估计值以及二进制数据的频率和百分比。
在 104 例患者中，GeneXpert 共检测到 10 例（9.62%）（95%置信区间为 3.94-15.26）结核病阳性病例。在所有阳性病例中，有 6 例（60%）为男性，4 例（40%）为女性，有 1 例（10%）为利福平耐药病例。
我们的研究发现，疑似病例中肺结核的患病率与报告的文献相似。
耐多药；核酸扩增检测；肺结核。
