NHC Key Laboratory of Pneumoconiosis, Key Laboratory of Prophylaxis and Treatment and Basic Research of Respiratory Diseases of Shanxi Province, Department of Respiratory and Critical Care Medicine, First Affiliated Hospital of Shanxi Medical University, Taiyuan, P.R. China.
Department of clinical inspection, Eleventh Affiliated Hospital of Shanxi Medical University, Lvliang, P.R. China.
Pol J Microbiol. 2022 Feb 23;71(1):27-34. doi: 10.33073/pjm-2022-002.
is an opportunistic fungus that can cause severe and potentially fatal pneumonia (PCP) in immunodeficient patients. In this study, we investigated the genetic polymorphisms of at eight different loci, including six nuclear genes (ITS, 26S rRNA, , , and β-Tub) and two mitochondrial genes (mtLSU-rRNA and ) in three PCP cases, including two patients with HIV infection and one without HIV infection in Shanxi Province, P.R. China. The gene targets were amplified by PCR followed by sequencing of plasmid clones. The HIV-negative patient showed a coinfection with two genotypes of at six of the eight loci sequenced. Of the two HIV-positive patients, one showed a coinfection with two genotypes of at the same two of the six loci as in the HIV-negative patient, while the other showed a single infection at all eight loci sequenced. None of the three drug target genes ( and ) showed mutations known to be potentially associated with drug resistance. This is the first report of genetic polymorphisms of in PCP patients in Shanxi Province, China. Our findings expand our understanding of the genetic diversity of in China.
是一种机会性真菌，可导致免疫功能低下的患者发生严重且可能致命的肺炎（PCP）。在这项研究中，我们调查了 8 个不同位点的 遗传多态性，包括 6 个核基因（ITS、26S rRNA、、、和β-Tub）和 2 个线粒体基因（mtLSU-rRNA 和 ），涉及山西省的 3 例 PCP 病例，包括 2 例 HIV 感染患者和 1 例非 HIV 感染患者。通过 PCR 扩增基因靶标，然后对质粒克隆进行测序。HIV 阴性患者在 8 个测序位点中的 6 个位点显示与两种基因型的 共感染。在 2 例 HIV 阳性患者中，1 例在与 HIV 阴性患者相同的 2 个 6 个位点上显示与两种基因型的 共感染，而另 1 例在所有 8 个测序位点上均显示单一感染。这 3 个药物靶基因（和）均未显示与耐药性相关的潜在突变。这是中国山西省首例关于 PCP 患者的 遗传多态性的报道。我们的研究结果扩展了我们对中国 遗传多样性的认识。
