Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Gastroenterology Surgical Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Pol J Microbiol. 2022 Feb 27;71(1):35-42. doi: 10.33073/pjm-2022-003.
To identify the associations between different genotypes of TLR9 -1486T/C (rs187084) with gastric cancer patients and reveal their relation to virulence genes (, , and ). Patients with gastric cancer were recruited to our study, diagnosed both endoscopically and histopathologically. were isolated from gastric samples by culture and PCR amplification of the gene. Virulence genes , , , and were detected by multiplex PCR. Blood samples were used for genotyping of TLR9 -1486T/C (rs187084) by PCR-RFLP. Out of 132 patients with gastric cancer, 106 (80.3%) were positive for . A similar number of healthy participants was recruited as controls. The prevalence of , and genes among was 90.6%, 70.8%, 83.0%, and 95.3%, respectively. The gene alleles had a prevalence of 95.3% for , 52.8% for , and 42.5% for . The CC genotype of TLR9 -1486T/C had a significantly higher frequency in gastric cancer patients when compared to healthy participants ( = 0.045). Furthermore, the CC genotype demonstrated a significant association with strains carrying , , and virulence genes ( = 0.021, = 0.049, and = 0.048 respectively). Patients with CC genotype of TLR9 -1486T/C (rs187084) might be at higher risk for the development of gastric cancer, and its co-existence with strains carrying , , or virulence genes might have a synergistic effect in the development of gastric cancer. Further studies on a wider scale are recommended.
为了确定 TLR9-1486T/C（rs187084）不同基因型与胃癌患者的关联，并揭示它们与毒力基因（，，和）的关系。我们招募了胃癌患者进行这项研究，通过内镜和组织病理学诊断。通过培养和基因的 PCR 扩增从胃样本中分离出。通过多重 PCR 检测毒力基因，，，和。使用血液样本通过 PCR-RFLP 对 TLR9-1486T/C（rs187084）进行基因分型。在 132 例胃癌患者中，有 106 例（80.3%）为阳性。招募了相同数量的健康参与者作为对照。在中，基因的发生率分别为 90.6%、70.8%、83.0%和 95.3%。基因等位基因的发生率分别为 95.3%、52.8%和 42.5%。与健康参与者相比，TLR9-1486T/C 的 CC 基因型在胃癌患者中具有更高的频率（=0.045）。此外，CC 基因型与携带，，和毒力基因的菌株显著相关（=0.021，=0.049，=0.048）。携带 TLR9-1486T/C（rs187084）的 CC 基因型的患者发生胃癌的风险可能更高，并且其与携带，，或毒力基因的菌株共存可能在胃癌的发展中具有协同作用。建议进行更大规模的进一步研究。
