Amin Mansour, Shayesteh Ali Akbar, Serajian Amirarsalan
Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Research Center for Infectious Diseases of Digestive System, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Iran J Microbiol. 2019 Jun;11(3):198-205.
is a Gram-negative spiral-shaped bacterium that contaminates more than half of the world's inhabitants, and infection with this bacterium is associated with some gastric disorders. Also, 5% to 10% of genes are specific to this bacterium and many bacterial virulence factors fall into this group. The and are among important virulence factors of .
A gastric biopsy specimen was taken from 341 gastric patients and cultivated on a Colombia agar plate, containing various antibiotics, such as vancomycin, amphotericin B, and trimethoprim & polymyxin B, and incubated for 3 to 10 days under microaerophilic conditions at 37°C. PCR was used to detect the and genes.
In this study, 131 isolates were identified as . The prevalence of and were 74%, 100%, 92.4% and 96.2%, respectively. The correlation between the clinical forms of the disease and the virulence genes were analyzed by statistical tests and no significant correlation was found.
The obtained results are similar to some studies conducted in different parts of the world and is different in other cases. This discrepancy is due to the difference in the type of gastric disorders, sample size and methodology.
[细菌名称]是一种革兰氏阴性螺旋形细菌，全球半数以上人口受到其感染，这种细菌感染与一些胃部疾病有关。此外，该细菌5%至10%的基因是其特有的，许多细菌毒力因子都属于这一组。[细菌名称]的[毒力因子名称1]和[毒力因子名称2]属于重要毒力因子。
从341名胃部疾病患者身上采集胃活检标本，接种于含有万古霉素、两性霉素B、甲氧苄啶和多粘菌素B等多种抗生素的哥伦比亚琼脂平板上，在37℃微需氧条件下培养3至10天。采用聚合酶链反应（PCR）检测[毒力因子名称1]和[毒力因子名称2]基因。
本研究中，131株分离菌被鉴定为[细菌名称]。[毒力因子名称1]和[毒力因子名称2]的检出率分别为74%、100%、92.4%和96.2%。通过统计学检验分析疾病临床类型与毒力基因之间的相关性，未发现显著相关性。
所得结果与世界不同地区进行的一些研究相似，在其他情况下则有所不同。这种差异是由于胃部疾病类型、样本量和方法的不同所致。
