School of Biomedical Engineering, Shanghai Jiao Tong University;
School of Biomedical Engineering, Shanghai Jiao Tong University.
J Vis Exp. 2022 May 10(183). doi: 10.3791/63770.
Characterization of biomechanical properties of soft biological tissues is important to understand the tissue mechanics and explore the biomechanics-related mechanisms of disease, injury, and development. The mechanical testing method is the most straightforward way for tissue characterization and is considered as verification for in vivo measurement. Among the many ex vivo mechanical testing techniques, the indentation test provides a reliable way, especially for samples that are small, hard to fix, and viscoelastic such as brain tissue. Magnetic resonance elastography (MRE) is a clinically used method to measure the biomechanical properties of soft tissues. Based on shear wave propagation in soft tissues recorded using MRE, viscoelastic properties of soft tissues can be estimated in vivo based on wave equation. Here, the viscoelastic properties of gelatin phantoms with two different concentrations were measured by MRE and indentation. The protocols of phantom fabrication, testing, and modulus estimation have been presented.
生物软组织的生物力学特性的表征对于理解组织力学和探索与生物力学相关的疾病、损伤和发育机制非常重要。力学测试方法是组织表征最直接的方法，被认为是体内测量的验证方法。在众多的离体力学测试技术中，压痕测试提供了一种可靠的方法，特别是对于脑组织等小而难以固定和粘弹性的样本。磁共振弹性成像（MRE）是一种用于测量软组织生物力学特性的临床方法。基于 MRE 记录的软组织结构中剪切波的传播，可以根据波动方程在体内估计软组织结构的粘弹性特性。在这里，通过 MRE 和压痕法测量了两种不同浓度明胶模型的粘弹性特性。介绍了模型制备、测试和模量估计的方案。
