College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, China; Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Sino-Danish College, University of Chinese Academy of Sciences, Beijing 101408, China.
Environ Int. 2022 Jul;165:107314. doi: 10.1016/j.envint.2022.107314. Epub 2022 May 20.
There is growing evidence that human gut microbiota can metabolize arsenic (As); however, which bacteria play roles in this metabolism is unclear. In this study, we measured the abilities of 21 human gut bacteria strains from diverse clades to adsorb and transform As using in vitro method with the aim of determining which bacteria play a role in As metabolism. Seven strains showed high biosorption of As, ranging from 20.1 to 29.8%, which was attributed to functional groups on the bacterial surfaces, such as hydroxyl, amino, and carboxyl groups. Moreover, six of these seven strains were versatile, as they also had roles in reducing As(V) to As(III), which is mainly regulated by the arsC gene. Escherichia coli had the strongest tolerance to As and the highest reducing ability, with a value of 71.04-73.13 µM As/h. This study reveals that gut bacteria play essential roles in As biosorption and biotransformation, and provides a better understanding of which strains are involved, which has implications for the regulation of As toxicity-based gut bacteria and provides basic data for regulating arsenic to human health.
越来越多的证据表明，人类肠道微生物群可以代谢砷（As）；然而，哪些细菌在这种代谢中起作用尚不清楚。在这项研究中，我们使用体外方法测量了来自不同进化枝的 21 个人类肠道细菌菌株吸附和转化 As 的能力，目的是确定哪些细菌在 As 代谢中起作用。七种菌株表现出对 As 的高生物吸附能力，范围从 20.1%到 29.8%，这归因于细菌表面的功能基团，如羟基、氨基和羧基。此外，这七种菌株中有六种是多功能的，因为它们还具有将 As(V)还原为 As(III)的作用，这主要受 arsC 基因调控。大肠杆菌对 As 的耐受性最强，还原能力最高，值为 71.04-73.13 µM As/h。本研究揭示了肠道细菌在 As 的生物吸附和生物转化中起着重要作用，并更好地了解了哪些菌株参与其中，这对基于肠道细菌调节 As 毒性具有重要意义，并为调节砷对人类健康的影响提供了基础数据。
