Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Provincial Key Laboratory of Agricultural & Rural Pollution Abatement and Environmental Safety, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
Guangdong Testing Institute of Product Quality Supervision, Foshan 528300, China.
J Hazard Mater. 2024 Jul 5;472:134623. doi: 10.1016/j.jhazmat.2024.134623. Epub 2024 May 14.
This study aimed to investigate the impact of arsenic stress on the gut microbiota of a freshwater invertebrate, specifically the apple snail (Pomacea canaliculata), and elucidate its potential role in arsenic bioaccumulation and biotransformation. Waterborne arsenic exposure experiments were conducted to characterize the snail's gut microbiomes. The results indicate that low concentration of arsenic increased the abundance of gut bacteria, while high concentration decreased it. The dominant bacterial phyla in the snail were Proteobacteria, Firmicutes, Bacteroidota, and Actinobacteriota. In vitro analyses confirmed the critical involvement of the gut microbiota in arsenic bioaccumulation and biotransformation. To further validate the functionality of the gut microbiota in vivo, antibiotic treatment was administered to eliminate the gut microbiota in the snails, followed by exposure to waterborne arsenic. The results demonstrated that antibiotic treatment reduced the total arsenic content and the proportion of arsenobetaine in the snail's body. Moreover, the utilization of physiologically based pharmacokinetic modeling provided a deeper understanding of the processes of bioaccumulation, metabolism, and distribution. In conclusion, our research highlights the adaptive response of gut microbiota to arsenic stress and provides valuable insights into their potential role in the bioaccumulation and biotransformation of arsenic in host organisms. ENVIRONMENTAL IMPLICATION: Arsenic, a widely distributed and carcinogenic metalloid, with significant implications for its toxicity to both humans and aquatic organisms. The present study aimed to investigate the effects of As on gut microbiota and its bioaccumulation and biotransformation in freshwater invertebrates. These results help us to understand the mechanism of gut microbiota in aquatic invertebrates responding to As stress and the role of gut microbiota in As bioaccumulation and biotransformation.
本研究旨在探讨砷胁迫对淡水贝类（苹果螺（Pomacea canaliculata））肠道微生物群的影响，并阐明其在砷生物积累和生物转化中的潜在作用。进行了水砷暴露实验以表征螺的肠道微生物组。结果表明，低浓度砷增加了肠道细菌的丰度，而高浓度则降低了其丰度。螺体内的主要细菌门是变形菌门、厚壁菌门、拟杆菌门和放线菌门。体外分析证实了肠道微生物群在砷生物积累和生物转化中的关键作用。为了进一步验证肠道微生物群在体内的功能，用抗生素处理消除螺体内的肠道微生物群，然后暴露于水砷中。结果表明，抗生素处理降低了螺体内的总砷含量和砷甜菜碱的比例。此外，生理基于药代动力学模型的利用提供了对生物积累、代谢和分布过程的更深入理解。总之，我们的研究强调了肠道微生物群对砷胁迫的适应性反应，并为它们在宿主生物砷生物积累和生物转化中的潜在作用提供了有价值的见解。环境意义：砷是一种广泛分布且具有致癌性的类金属，对人类和水生生物都具有重要的毒性。本研究旨在探讨砷对淡水无脊椎动物肠道微生物群及其生物积累和生物转化的影响。这些结果有助于我们了解肠道微生物群在水生无脊椎动物对砷胁迫的反应机制，以及肠道微生物群在砷生物积累和生物转化中的作用。
