Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada; Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada; Waterloo Water Institute, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada.
Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada; Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada.
J Colloid Interface Sci. 2022 Oct;623:883-896. doi: 10.1016/j.jcis.2022.05.084. Epub 2022 May 17.
A robust method to prepare silver nanoclusters (AgNCs) inside a methacrylic acid-ethyl acrylate (MAA-EA) nanogel is proposed, where AgNCs were produced within the nanogel scaffold via UV-photoreduction. The impact of UV irradiation time on the formation of AgNCs and their application in biolabeling and antimicrobial properties were examined. The AgNCs formation is described by two stages; (1) Ag (n = 2-8) nanoclusters formation between 0 and 25 min, and (2) larger silver nanoparticles (AgNPs) formed via aggregation inside the nanogel. The antimicrobial performance depended on the size and concentration of silver ions (Ag). A maximum inhibitory concentration (MIC) of 1.1 ppm was observed for antimicrobial test with yeast, and a MIC of 11 and 22 ppm was recorded for Escherichia. coli and Staphylococcus aureus respectively. Combining with the green illumination property of AgNCs (emitted at 525 nm) with dead yeast, it could be used for biolabeling. By tuning the size through photoirradiation, the nanogel templated AgNCs is a promising candidate for antimicrobial and biolabeling applications.
提出了一种在甲基丙烯酸-丙烯酸乙酯（MAA-EA）纳米凝胶内制备银纳米簇（AgNCs）的稳健方法，其中通过 UV 光还原在纳米凝胶支架内生成 AgNCs。考察了 UV 照射时间对 AgNCs 形成及其在生物标记和抗菌性能中应用的影响。AgNCs 的形成可以分为两个阶段描述：（1）在 0 到 25 分钟之间形成 Ag（n=2-8）纳米簇，（2）通过纳米凝胶内的聚集形成更大的银纳米粒子（AgNPs）。抗菌性能取决于银离子（Ag）的大小和浓度。在对酵母进行的抗菌试验中，观察到最大抑制浓度（MIC）为 1.1ppm，对大肠杆菌和金黄色葡萄球菌的 MIC 分别为 11 和 22ppm。AgNCs（在 525nm 处发射）与死亡酵母的绿色发光特性相结合，可用于生物标记。通过光辐照调节尺寸，纳米凝胶模板化的 AgNCs 是抗菌和生物标记应用的有前途的候选物。
