College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, Shaanxi 710119, China.
College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, Shaanxi 710119, China.
Food Res Int. 2022 Oct;160:111680. doi: 10.1016/j.foodres.2022.111680. Epub 2022 Jul 14.
Bacterial infection has become an important factor affecting human health, and the increasing antibiotic resistance has seriously hindered the treatment of infectious diseases. This study aimed to explore a novel nanotechnology that combines silver with glutathione (GSH) to form antibacterial nanoclusters, GSH@AgNCs. The composite was characterized using a UV fluorescence spectrophotometer, high-resolution transmission electron microscopy (HR-TEM), particle size-zeta potential, fourier transform infrared (FTIR), X-ray photoelectron spectrometer (XPS), thermal gravimetric analysis (TGA), and X-ray diffraction analysis (XRD). This study examined the inhibitory effect of GSH@AgNCs on the bacterial growth and biofilm formation of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), as well as its antibacterial mechanisms. The results indicated that GSH@AgNCs were more successful in restricting E. coli than S. aureus. The bacterial membrane exposed to GSH@AgNCs was damaged irreversibly, presenting cytoplasm leakage, membrane depolarization, ATPase activity decline, and cell degeneration. In addition, at low concentration (1/8 MIC), GSH@AgNCs significantly inhibited the formation of biofilms and damaged mature biofilms, reducing the viable cells. This study demonstrated that GSH@AgNCs effectively hindered the proliferation of foodborne Gram-positive bacteria (S. aureus) and Gram-negative bacteria (E. coli), providing new feasibility for applying organic composite nanomaterials and nanotechnology in the food industry.
细菌感染已成为影响人类健康的重要因素，而抗生素耐药性的不断增加严重阻碍了传染病的治疗。本研究旨在探索一种将银与谷胱甘肽（GSH）结合形成抗菌纳米团簇的新型纳米技术，即 GSH@AgNCs。使用紫外荧光分光光度计、高分辨率透射电子显微镜（HR-TEM）、粒径-动电电位、傅里叶变换红外光谱（FTIR）、X 射线光电子能谱（XPS）、热重分析（TGA）和 X 射线衍射分析（XRD）对复合材料进行了表征。本研究考察了 GSH@AgNCs 对大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）的细菌生长和生物膜形成的抑制作用及其抗菌机制。结果表明，GSH@AgNCs 对 E. coli 的抑制作用更为成功。暴露于 GSH@AgNCs 的细菌膜被不可逆地破坏，呈现细胞质泄漏、膜去极化、ATP 酶活性下降和细胞退化。此外，在低浓度（1/8 MIC）下，GSH@AgNCs 可显著抑制生物膜的形成并破坏成熟的生物膜，减少存活细胞。本研究表明，GSH@AgNCs 可有效抑制食源性病原体革兰氏阳性菌（S. aureus）和革兰氏阴性菌（E. coli）的增殖，为有机复合纳米材料和纳米技术在食品工业中的应用提供了新的可行性。
