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新型严重急性呼吸系统综合症冠状病毒 2 分子检测方法

Novel Assays for Molecular Detection of Severe Acute Respiratory Syndrome Coronavirus 2.

机构信息

Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, 3400 Spruce Street, 4th Floor Gates Building, Philadelphia, PA 19104, USA; Clinical Microbiology Laboratory, Hospital of the University of Pennsylvania, Philadelphia, PA, USA. Electronic address: https://twitter.com/KGRodinoPhD.

Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Infectious Disease Diagnostics Laboratory, Children's Hospital of Philadelphia, Main Building 5NW91, 3401 Civic Center Boulevard, Philadelphia, PA 19104, USA.

出版信息

Clin Lab Med. 2022 Jun;42(2):299-307. doi: 10.1016/j.cll.2022.02.004. Epub 2022 Mar 9.

Abstract

From the onset of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)/COVID-19 pandemic, there has been a major emphasis on molecular laboratory tests for the virus. Shortages in various testing supplies, the desire to increase testing capacity, and a push to make point-of-care or home-based testing available have fostered considerable innovation for SARS-CoV-2 molecular diagnostics, advancements likely to be applicable to other diagnostic uses. The authors attempt to cover some of the most compelling novel types of molecular assays or novel approaches in adapting established molecular methodologies for SARS-CoV-2 detection or characterization.

摘要

从严重急性呼吸综合征冠状病毒 2(SARS-CoV-2)/COVID-19 大流行开始,人们就一直非常重视针对该病毒的分子实验室检测。各种检测用品的短缺、提高检测能力的愿望以及推动实施即时检测或家庭检测,都为 SARS-CoV-2 分子诊断带来了相当大的创新,这些进展可能适用于其他诊断用途。作者试图介绍一些最引人注目的新型分子检测方法,或为 SARS-CoV-2 检测或特征分析而改编现有分子方法的新方法。

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