Thompson Alec T, Garrett Kayla B, Kirchgessner Megan, Ruder Mark G, Yabsley Michael J
Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, 30602, USA.
Center for the Ecology of Infectious Diseases, Odum School of Ecology, University of Georgia, 30602, USA.
Int J Parasitol Parasites Wildl. 2022 May 19;18:180-183. doi: 10.1016/j.ijppaw.2022.05.005. eCollection 2022 Aug.
In 2017, clinical disease and mortality in cattle associated with Ikeda was reported in Virginia, U.S. The exotic tick, is a competent vector for this species. White-tailed deer () are commonly infested with in the eastern U.S. and are also infected with several genotypes of piroplasms such as a sp. (often called like), , and sp. H10. However, it is currently unknown if deer are susceptible to and can act as potential hosts. In this study, we tested 552 white-tailed deer samples from the southeastern U.S. to determine the presence of . We used a PCR-RFLP to test 293 (53%) of these samples to distinguish between piroplasm genera. A total of 189 white-tailed deer were positive with 47 were positive with , and 57 did not amplify. Because this assay does not determine species, we sequenced 30 random samples targeting a fragment of the 18S rRNA gene. Although a high diversity of and spp. were detected, none were . All 552 samples were then screened with a specific real-time PCR protocol, but none were positive for Our data suggests that white-tailed deer are commonly infected with piroplasm species but not .
2017年，美国弗吉尼亚州报告了与池田（Ikeda）相关的牛临床疾病和死亡率。这种外来蜱是该物种的有效传播媒介。白尾鹿（Odocoileus virginianus）在美国东部通常受到这种蜱的侵扰，并且还感染了几种梨形虫基因型，如一种泰勒虫（Theileria sp.）（通常称为Theileria luwenshuni样）、环形泰勒虫（T. annulata）和泰勒虫H10种。然而，目前尚不清楚鹿是否易感染池田泰勒虫（Theileria ikedai）并能否作为潜在宿主。在本研究中，我们检测了来自美国东南部的552份白尾鹿样本，以确定池田泰勒虫的存在情况。我们使用PCR-RFLP检测了其中293份（53%）样本，以区分梨形虫属。共有189只白尾鹿对泰勒虫属呈阳性，47只对环形泰勒虫呈阳性，57只未扩增。由于该检测方法不能确定物种，我们对30个随机样本进行测序，靶向18S rRNA基因的一个片段。尽管检测到了高度多样的泰勒虫属和其他种，但没有一个是池田泰勒虫。然后，我们用池田泰勒虫特异性实时PCR方案对所有552份样本进行筛查，但没有一个样本对池田泰勒虫呈阳性。我们的数据表明，白尾鹿通常感染梨形虫物种，但未感染池田泰勒虫。
