Thompson Alec T, Dominguez Kristen, Cleveland Christopher A, Dergousoff Shaun J, Doi Kandai, Falco Richard C, Greay Telleasha, Irwin Peter, Lindsay L Robbin, Liu Jingze, Mather Thomas N, Oskam Charlotte L, Rodriguez-Vivas Roger I, Ruder Mark G, Shaw David, Vigil Stacey L, White Seth, Yabsley Michael J
Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, United States.
Center for the Ecology of Infectious Diseases, Odum School of Ecology, University of Georgia, Athens, GA, United States.
Front Vet Sci. 2020 Mar 13;7:141. doi: 10.3389/fvets.2020.00141. eCollection 2020.
(Acari: Ixodidae), the Asian longhorned tick, is native to East Asia, but has become established in Australia and New Zealand, and more recently in the United States. In North America, there are other native species that share similar morphological characteristics and can be difficult to identify if the specimen is damaged. The goal of this study was to develop a cost-effective and rapid molecular diagnostic assay to differentiate between exotic and native species to aid in ongoing surveillance of within the United States and help prevent misidentification. We demonstrated that restriction fragment length polymorphisms (RFLPs) targeting the 16S ribosomal RNA and the cytochrome oxidase subunit I () can be used to differentiate from the other species found in North America. Furthermore, we show that this RFLP assay can be applied to species endemic to other regions of the world for the rapid identification of damaged specimens. The work presented in this study can serve as the foundation for region specific PCR-RFLP keys for and other tick species and can be further applied to other morphometrically challenging taxa.
亚洲长角蜱（蜱螨目：硬蜱科）原产于东亚，但已在澳大利亚和新西兰立足，最近在美国也有发现。在北美，还有其他本地物种具有相似的形态特征，如果标本受损，可能难以识别。本研究的目的是开发一种经济高效且快速的分子诊断检测方法，以区分外来物种和本地物种，协助美国境内的持续监测，并有助于防止误识别。我们证明，针对16S核糖体RNA和细胞色素氧化酶亚基I（）的限制性片段长度多态性（RFLP）可用于区分亚洲长角蜱与北美发现的其他物种。此外，我们表明这种RFLP检测方法可应用于世界其他地区特有的蜱种，以快速鉴定受损标本。本研究中提出的工作可为亚洲长角蜱和其他蜱种的区域特异性PCR-RFLP鉴定方法奠定基础，并可进一步应用于其他形态测量具有挑战性的分类群。
