某些专性噬菌体能形成形态和遗传多样性。

Morphogenesis and Genetic Diversity of Some Virulent Phages Specific for .

出版信息

Pak J Biol Sci. 2022 Jan;25(5):433-443. doi: 10.3923/pjbs.2022.433.443.

DOI:10.3923/pjbs.2022.433.443

Abstract

Background and Objective: Phosphorus (P) is one of the most limiting nutrients for plant growth. Phosphorus deficiency is limiting crop production in many agricultural soils worldwide. The application of phosphorus solubilizing bacteria (PSB) to soils can replace or partially reduce using of inorganic P fertilizers. A bacteriophage, or phage, is a virus that infects a bacterial cell, taking over the host cell's genetic material. The four phages were propagated, purified, studied for the morphological properties, finally studying the genetic diversity. Materials and Methods: Obtained, examined the efficiency and identification of bacteria for solubilizing phosphorus. Isolation, studying the properties and studying genetic diversity. Results: Four virulent phages (Bv1, Bv2, Bv3 and Bv4) specific for Bacillus velezensis were isolated from the Egyptian soil. The Bacillus phages were purified by alternative low and high-speed centrifugation methods. Electron micrographs showed that phages appeared to be a member of the Siphoviridae family based on their structure and particle morphology (the particles have a head and long non-contractile tail). Sodium Dodecyl Sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) technique was performed to determine the properties of viral proteins. It was found that the Bv1 virus had five structural proteins, while Bv2 and Bv3 virus had eight structural proteins and finally, the Bv4 virus had ten structural proteins. The purity and quantity of isolated DNAs were determined spectrophotometrically. Data showed that the concentration of Bv1 DNA was 0.75 μg, Bv2 DNA and Bv3 DNA was 0.60 μg and finally Bv4 DNA 0.55 μg μL1. The analysis of genetic material of B. velezensis phages was determined based on both the ISSR-PCR technique and the effect of restriction enzymes. Data showed different amplification patterns with all phages. Conclusion: The bacteriophages of B. velezensis were isolated from soil, propagated, purified, study some of its properties.

摘要

磷（P）是植物生长最主要的限制营养元素之一。在全球许多农业土壤中，磷缺乏限制了作物的产量。将解磷细菌（PSB）应用于土壤中可以替代或部分减少无机磷肥料的使用。噬菌体，又称 phage，是一种感染细菌细胞的病毒，接管宿主细胞的遗传物质。对这四种噬菌体进行了繁殖、纯化、形态学特性研究，最后研究了遗传多样性。

材料与方法

获得、检测了用于溶解磷的细菌的效率和鉴定。分离、研究了特性和遗传多样性。

结果

从埃及土壤中分离到了四种针对巴氏芽孢杆菌的烈性噬菌体（Bv1、Bv2、Bv3和 Bv4）。通过交替的低速和高速离心方法对芽孢杆菌噬菌体进行了纯化。电子显微镜照片显示，根据其结构和颗粒形态（颗粒具有头部和非收缩的长尾），噬菌体似乎属于 长尾噬菌体科家族。进行了十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）技术来确定病毒蛋白的性质。结果发现，Bv1病毒有 5 种结构蛋白，而 Bv2和 Bv3病毒有 8 种结构蛋白，最后 Bv4病毒有 10 种结构蛋白。通过分光光度法确定分离 DNA 的纯度和数量。数据显示，Bv1DNA 的浓度为 0.75 μg，Bv2DNA 和 Bv3DNA 的浓度为 0.60 μg，最后 Bv4DNA 的浓度为 0.55 μgμL1。基于 ISSR-PCR 技术和限制酶的作用，对巴氏芽孢杆菌噬菌体的遗传物质进行了分析。数据显示，所有噬菌体都表现出不同的扩增模式。