建立 Taqman 实时荧光 PCR 方法鉴定长角血蜱（蜱螨目：硬蜱科）。
Development of a Taqman Real-Time PCR for the Identification of Haemaphysalis longicornis (Acari: Ixodidae).
机构信息
Department of Microbiology, University of Massachusetts, Amherst MA, USA.
Department of Microbiology, University of Massachusetts, Amherst, MA, USA.
出版信息
J Med Entomol. 2022 Jul 13;59(4):1484-1487. doi: 10.1093/jme/tjac074.
Haemaphysalis longicornis Neumann, a vector of various pathogens with medical and veterinary importance, is a recent invasive species in the United States. Like many tick species, discerning H. longicornis from congeners can be a challenge. To overcome the difficulty of morphological identification, a Taqman quantitative real-time PCR based on the internal transcribed spacer gene (ITS2) was developed for quick and accurate identification of H. longicornis with a detection limit of as low as 19.8 copies. We also applied the assay to 76,004 archived ticks and found 37 ticks were H. longicornis. One H. longicornis was submitted from Warren, Somerset County, New Jersey in June 2015, 2 yr earlier than the initial report from the United States. None of these 37 H. longicornis was positive for Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, B. miyamotoi, B. mayonii, Babesia microti, or Ehrlichia muris-like agent.
长角血蜱（Haemaphysalis longicornis）是一种具有医学和兽医学重要性的多种病原体的载体，是美国最近入侵的物种。与许多蜱种一样，长角血蜱与同属物种的区分可能具有挑战性。为了克服形态鉴定的困难，我们开发了一种基于内部转录间隔区基因（ITS2）的 Taqman 定量实时 PCR 方法，可快速准确地鉴定长角血蜱，检测限低至 19.8 拷贝。我们还将该检测方法应用于 76004 个存档的蜱虫中，发现 37 个蜱虫为长角血蜱。其中 1 只长角血蜱于 2015 年 6 月从新泽西州萨默塞特县的沃伦提交，比美国最初的报告早了 2 年。这 37 只长角血蜱中均未检测到嗜吞噬细胞无形体、伯氏疏螺旋体、无形体、马氏巴贝斯虫、微小巴贝斯虫或鼠埃立克体样病原体。
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