State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Food Chem. 2022 Oct 15;391:133280. doi: 10.1016/j.foodchem.2022.133280. Epub 2022 May 20.
Triacylglycerol (TAG) regioisomers containing palmitic acid (16:0) was identified using ultra-performance supercritical fluid chromatography and quadrupole time-of-flight mass spectrometry (UPSFC-Q-TOF-MS) and quantified using calibration curve method and calculation equation method. There were negative linear correlation between [R]/[R+[R] and content of sn-A-B-A (%) for AAB/ABA type TAGs, [R]/[R+[R]+[R] and content of fatty acid (FA) at sn-2 position (%) for BAC/ABC/ACB type TAGs. The difference between calculation equation and standard curve method was acceptable. The TAG regioisomers in human milk, mammalian milk, lard and fish oil were identified and quantified using the developed methods. This study provided a reliable and facile method for analysis of the TAG regioisomers, which was capable of the selection of oil materials for infant formula production.
采用超高效超临界流体色谱-四级杆飞行时间质谱联用技术（UPSFC-Q-TOF-MS）对含有棕榈酸（16:0）的三酰基甘油(TAG)立体异构体进行了鉴定，并采用校准曲线法和计算方程法进行了定量分析。AAB/ABA 型 TAG 中 [R]/[R+[R]与 sn-A-B-A（%）含量呈负线性相关，BAC/ABC/ACB 型 TAG 中 [R]/[R+[R]+[R]与 sn-2 位脂肪酸（FA）含量（%）呈负线性相关。计算方程法与标准曲线法的差异是可以接受的。采用所建立的方法对人乳、哺乳动物乳、猪油和鱼油中的 TAG 立体异构体进行了鉴定和定量分析。本研究为 TAG 立体异构体的分析提供了一种可靠、简便的方法，为婴儿配方奶粉生产中油脂原料的选择提供了依据。
