Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China.
Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Oct 15;279:121427. doi: 10.1016/j.saa.2022.121427. Epub 2022 May 27.
This work innovatively integrated the peroxidase-mimicking activity and red emission property of Fe@PCN-222 framework, designed a cascade reaction system for dual-mode glucose sensing. The Fe doping significantly improved the catalytic activity of Fe@PCN-222 that can oxidize the substrate o-phenylenediamine (OPD) to generate diminophenazine (DAP) with emission at 566 nm in the presence of HO. Similarly, the Fe@PCN-222 was used to catalyze the colorless TMB to produce blue oxidized TMB (oxTMB) showed absorption at 652 nm. When coupled with glucose oxidase (GOx), the linear ranges of ratiometric fluorescence mode and colorimetric mode for glucose sensing were 1-100 and 10-300 μM, respectively. And the limits of detection (LOD) of 0.78 and 2.41 μM for two modes were obtained, respectively. In addition, the practicability of Fe@PCN-222 nanozyme-based cascade reaction system for detection of glucose in human serum and saliva samples was successfully investigated. It is of great importance to integrate more functions into one skeleton to achieve dual-mode and optimal-performance sensing for expanding potential applications.
这项工作创新性地整合了 Fe@PCN-222 骨架的过氧化物酶模拟活性和红色发射特性，设计了用于双模式葡萄糖传感的级联反应系统。Fe 掺杂显著提高了 Fe@PCN-222 的催化活性，在 HO 的存在下，它可以氧化底物邻苯二胺 (OPD) 生成发射波长为 566nm 的二氨基吩嗪 (DAP)。同样，Fe@PCN-222 被用于催化无色 TMB 生成蓝色氧化 TMB（oxTMB），在 652nm 处有吸收。当与葡萄糖氧化酶 (GOx) 偶联时，比率荧光模式和比色模式的葡萄糖传感线性范围分别为 1-100 和 10-300μM，两种模式的检测限 (LOD) 分别为 0.78 和 2.41μM。此外，成功研究了基于 Fe@PCN-222 纳米酶的级联反应系统用于检测人血清和唾液样本中葡萄糖的实用性。将更多功能集成到一个骨架中以实现双模式和最佳性能传感，对于扩展潜在应用具有重要意义。
