State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin, 541004, People's Republic of China.
Mikrochim Acta. 2019 Apr 23;186(5):295. doi: 10.1007/s00604-019-3416-7.
A metal organic framework (MOF) of type Fe(III)-BTC (where BTC is 1,3,5-benzenetricarboxylic acid) was utilized to construct an integrated system for cascade colorimetric determination of glucose. The MOF performs a dual function in acting (a) as a peroxidase (POx) mimic, and (b) as a solid support for immobilization of glucose oxidase (GOx). The MOF was prepared by a one-pot method. Glucose is consumed while HO is produced during the enzymatic oxidation by GOx. In the presence of HO, the POx mimic catalytically oxidizes 3,3',5,5'-tetramethylbenzidine (TMB) to form a blue-green product. The absorbance of oxidized TMB (measured at 652 nm) increases linearly in the 5.0-100 μM glucose concentration range, and the detection limit is 2.4 μM. The GOx@Fe-BTC MOF was successfully applied to the determination of glucose in serum. Graphical abstract Schematic presentation of a bifunctional metal organic framework of type Fe-BTC for cascade (enzymatic and enzyme-mimicking) colorimetric determination of glucose. The Fe-BTC performs a dual function in acting as both a peroxidase mimic and support for immobilizing glucose oxidase. Using the integrated enzyme, a colorimetric method was successfully applied to one-step detection of glucose in human serum.
一种类型为 Fe(III)-BTC（BTC 是 1,3,5-苯三甲酸）的金属有机骨架（MOF）被用于构建级联比色测定葡萄糖的集成系统。MOF 具有双重功能，既可以作为过氧化物酶（POx）模拟物，又可以作为固定葡萄糖氧化酶（GOx）的固体支撑物。MOF 通过一锅法制备。在 GOx 的酶促氧化过程中，葡萄糖被消耗，HO 被产生。在 HO 的存在下，POx 模拟物催化氧化 3,3',5,5'-四甲基联苯胺（TMB）形成蓝绿色产物。氧化 TMB 的吸光度（在 652nm 处测量）在 5.0-100μM 葡萄糖浓度范围内呈线性增加，检测限为 2.4μM。GOx@Fe-BTC MOF 成功应用于血清中葡萄糖的测定。示意图 Fe-BTC 型双功能金属有机骨架用于级联（酶促和酶模拟）比色测定葡萄糖。Fe-BTC 既可以作为过氧化物酶模拟物，又可以作为固定葡萄糖氧化酶的载体。使用集成酶，成功地应用比色法一步检测人血清中的葡萄糖。
