Martin M F, Rochat H
Toxicon. 1986;24(11-12):1131-9. doi: 10.1016/0041-0101(86)90139-x.
A large scale procedure for purification of the toxins in the venom of the North African scorpion Androctonus australis has been developed. This procedure optimizes the sequence of the steps, leading to better yields of toxins and shortening of the time. It is possible to use 10-15 g batches of venom and also prevent cross-contamination of the toxins. High pressure liquid chromatography has been used to separate AaH I and I', two isotoxins which differ by one amino acid residue in position 17: valine or isoleucine. The procedure allows the characterization of a new toxin active in mice, AaH IV, which represents 0.6 and 2.5% of the venom weight and toxicity, respectively. This new component has been characterized as an alpha toxin made up of 61 amino acid residues and having a neutral isoelectric point.
已开发出一种大规模纯化北非蝎子黄肥尾蝎毒液中毒素的方法。该方法优化了步骤顺序，提高了毒素产量并缩短了时间。可以使用10 - 15克批次的毒液，还能防止毒素的交叉污染。高压液相色谱已用于分离AaH I和I'，这两种异毒素在第17位氨基酸残基上相差一个：缬氨酸或异亮氨酸。该方法可鉴定出一种对小鼠有活性的新毒素AaH IV，其分别占毒液重量和毒性的0.6%和2.5%。这种新成分已被鉴定为一种由61个氨基酸残基组成且具有中性等电点的α毒素。
