Romer Labs, Inc., 130 Sandy Drive, Newark, DE 19713, USA.
J AOAC Int. 2022 Oct 26;105(6):1652-1662. doi: 10.1093/jaoacint/qsac064.
Campylobacter is one of the leading causes of human bacterial gastroenteritis worldwide. Campylobacter infections are most often associated with the consumption of raw milk, undercooked poultry, and contaminated water.
The RapidChek®Campylobacter test system (PTM number 052201) was validated for the detection of Campylobacter jejuni, C. coli, and C. lari in raw ground chicken, chicken carcass rinse, and turkey carcass sponges.
The method uses a proprietary enrichment medium. Following aerobic enrichment, an immunochromatographic test strip is inserted into the tube containing the enrichment, developed for 20 min, and interpreted. Campylobacter-inoculated food samples were tested by the method, as well as the USDA/FSIS cultural reference method; Isolation and Identification of Campylobacter jejuni/coli/lari from Poultry Rinse, Sponge and Raw Product Samples MLG 41.04. The candidate method was also confirmed by an alternative cultural method. The RapidChek method was tested with 50 Campylobacter strains comprised of C. jejuni, C. coli, and C. lari, and 30 non-target strains.
A total of 80 low-level spiked samples were tested by both methods in the study. The candidate method yielded 49 presumptive positives: all presumptive results were confirmed culturally. The reference method produced a total of 41 confirmed positive results. No difference between the alternate confirmation method and reference confirmation method was observed. Probability of detection analysis demonstrated no significant differences in the number of positive samples detected by the candidate method and cultural reference method. The RapidChek method detected all 50 Campylobacter strains and none of the 30 non-target strains, including Campylobacter spp. other than C. jejuni, C. coli, and C. lari.
The candidate method performed as well as the reference method in the detection of C. jejuni, C. coli, and C. lari in raw ground chicken, chicken carcass rinse, and turkey carcass sponges.
Aerobic enrichment of selected matrixes for 48 h yielded reliable presumptive results for Campylobacter.
空肠弯曲菌是全世界导致人类细菌性肠胃炎的主要原因之一。空肠弯曲菌感染通常与食用生牛奶、未煮熟的家禽和受污染的水有关。
验证 RapidChek®弯曲菌检测系统（PTM 编号 052201）用于检测生鸡肉碎、鸡胴体冲洗液和火鸡胴体海绵中的空肠弯曲菌、大肠弯曲菌和 C. lari。
该方法使用专有增菌培养基。在有氧增菌后，将免疫层析测试条插入含有增菌液的管中，培养 20 分钟，然后进行判读。通过该方法以及美国农业部/食品安全检验局（USDA/FSIS）的文化参考方法对含弯曲菌的食品样本进行了测试；从禽类冲洗液、海绵和生产品样本中分离和鉴定空肠弯曲菌/大肠弯曲菌/ C. lari MLG 41.04。候选方法还通过替代培养方法得到了确认。该 RapidChek 方法用 50 株空肠弯曲菌、大肠弯曲菌和 C. lari 组成的弯曲菌菌株以及 30 株非目标菌株进行了测试。
在研究中，两种方法共测试了 80 个低水平加标样本。候选方法共产生 49 个推定阳性结果：所有推定结果均经培养确认。参考方法共产生 41 个确认阳性结果。替代确认方法与参考确认方法之间没有差异。检测概率分析表明，候选方法和文化参考方法检测到的阳性样本数量没有显著差异。RapidChek 方法检测到所有 50 株弯曲菌菌株，而 30 株非目标菌株均未检出，包括除空肠弯曲菌、大肠弯曲菌和 C. lari 以外的弯曲菌属菌株。
候选方法在生鸡肉碎、鸡胴体冲洗液和火鸡胴体海绵中检测空肠弯曲菌、大肠弯曲菌和 C. lari 的性能与参考方法相当。
对选定基质进行 48 小时有氧增菌可获得弯曲菌可靠的推定结果。
