School of Life and Health Science, Hunan University of Science and Technology, Xiangtan, 411201, Hunan, China.
Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, Hunan University of Science and Technology, Xiangtan, 411201, Hunan, China.
BMC Genom Data. 2022 May 31;23(1):39. doi: 10.1186/s12863-022-01058-6.
The influence of environmental factors, especially temperature, on sex ratio is of great significance to elucidate the mechanism of sex determination. However, the molecular mechanisms by which temperature affects sex determination remains unclear, although a few candidate genes have been found to play a role in the process. In this study, we conducted transcriptome analysis of the effects induced by high temperature on zebrafish during gonad differentiation period.
Totals of 1171, 1022 and 2921 differentially expressed genes (DEGs) between high temperature and normal temperature were identified at 35, 45 and 60 days post-fertilization (dpf) respectively, revealing that heat shock proteins (HSPs) and DNA methyltransferases (DNMTs) were involved in the heat-exposed sex reversal. The Gene Ontology (GO) terms and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway that were enriched in individuals after heat treatment included Fanconi anemia (FA) pathway, cell cycle, oocyte meiosis and homologous recombination.
Our study provides the results of comparative transcriptome analyses between high temperature and normal temperature, and reveals that the molecular mechanism of heat-induced masculinization in zebrafish is strongly related to the expression of HSPs and DNMTs and FA pathway during gonad differentiation.
环境因素，尤其是温度，对性别比例的影响对于阐明性别决定的机制具有重要意义。然而，尽管已经发现了一些候选基因在这个过程中起作用，但温度如何影响性别决定的分子机制仍不清楚。在这项研究中，我们对高温在斑马鱼性腺分化期诱导的影响进行了转录组分析。
在受精后 35、45 和 60 天，高温和正常温度之间分别鉴定出 1171、1022 和 2921 个差异表达基因（DEGs），表明热休克蛋白（HSPs）和 DNA 甲基转移酶（DNMTs）参与了热暴露导致的性别反转。经热处理后的个体中富集的基因本体（GO）术语和京都基因与基因组百科全书（KEGG）途径包括范可尼贫血（FA）途径、细胞周期、卵母细胞减数分裂和同源重组。
本研究提供了高温与正常温度之间比较转录组分析的结果，表明在性腺分化过程中，热诱导的雄性化的分子机制与 HSPs 和 DNMTs 以及 FA 途径的表达密切相关。
