Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, Shandong, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong, China.
Laboratory of Fisheries Oceanography, College of Fisheries, Ocean University of China, Qingdao, China.
Ecotoxicol Environ Saf. 2022 Mar 1;232:113250. doi: 10.1016/j.ecoenv.2022.113250. Epub 2022 Feb 1.
The sea temperature has been observed to chronically increase during the past decades, leaving unpredictable influences to the marine biological resources. Thus, it is of vital significance to study the biological responses of ocean inhabited organisms with the artificially stimulated heat stress environment. Cynoglossus semilaevis provides us with an ideal model to study the influence of chronic heat stress on the sexual differentiation in marine teleosts for its genetic sex determination (GSD) + environmental effected (EE) sex determination system. In this study, the comparative experiment was conducted employing heated seawater (HT group) and ambient seawater (CT group) to cultivate juvenile C. semilaevis respectively. Significant differences were exhibited in growth performance and a delayed germ cell development effect was found in pseudomales formed under chronic heat stress. Using transcriptome analysis, the transcription profile of 55 days post fertilization (dpf) and 100 dpf juveniles' gonads were studied. A total of 47 libraries were constructed with an average mapping rate of 94.63% after assembling. GO and KEGG enrichment were proceeded using DEGs screened out between (1) pseudomale gonads at 55 dpf and 100 dpf in HT and CT group (2) pseudomale and female gonads at 55 dpf and 100 dpf in HT and CT group. Terms and pathways involved in steroid stimulation, reproduction ability, germ cell proliferation et al. were shed light on. The expression pattern of 29 DEGs including amh, hsp90b1, pgr et al. were also provided to supplement the results of functional enrichment. Weighted gene co-expression networks analysis (WGCNA) was constructed and hspb8-like, histone H2A.V were exhibited to play vital roles in the heat-induced masculinization. Our findings facilitate the understanding for transcriptional variations in intensive masculinization cause by chronic heat stress of C. semilaevis and provide referable study of the influences on the teleosts in elevated sea temperature.
过去几十年来，海洋温度一直呈慢性上升趋势，对海洋生物资源产生了不可预测的影响。因此，研究海洋生物在人为刺激的热应激环境下的生物反应具有重要意义。半滑舌鳎提供了一个理想的模型，用于研究慢性热应激对海洋硬骨鱼类性别分化的影响，因为它具有遗传性别决定（GSD）+环境影响（EE）性别决定系统。在这项研究中，采用加热海水（HT 组）和环境海水（CT 组）分别培养幼年半滑舌鳎。结果表明，在慢性热应激下形成的假雄鱼的生长性能存在显著差异，并且出现了延迟的生殖细胞发育效应。利用转录组分析，研究了受精后 55 天（dpf）和 100 天（dpf）幼鱼性腺的转录谱。共构建了 47 个文库，组装后平均映射率为 94.63%。使用 DEGs 在 HT 和 CT 组（1）55 dpf 和 100 dpf 时的假雄鱼性腺之间，（2）55 dpf 和 100 dpf 时的假雄鱼和雌鱼性腺之间进行了 GO 和 KEGG 富集。揭示了类固醇刺激、生殖能力、生殖细胞增殖等相关术语和途径。还提供了包括 amh、hsp90b1、pgr 等在内的 29 个 DEG 的表达模式，以补充功能富集的结果。构建了加权基因共表达网络分析（WGCNA），结果表明 hspb8-like 和组蛋白 H2A.V 在热诱导雄性化中发挥重要作用。我们的研究结果有助于了解慢性热应激对半滑舌鳎强烈雄性化的转录变化，并为升高的海洋温度对硬骨鱼类的影响提供了有价值的研究。
Zotero 插件