Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States.
Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee 37996, United States.
Environ Sci Technol. 2022 Jun 21;56(12):8155-8166. doi: 10.1021/acs.est.2c01454. Epub 2022 Jun 1.
Anthropogenic organofluorine compounds are recalcitrant, globally distributed, and a human health concern. Although rare, natural processes synthesize fluorinated compounds, and some bacteria have evolved mechanisms to metabolize organofluorine compounds. sp. strain 273 grows with 1-fluorodecane (FD) and 1,10-difluorodecane (DFD) as carbon sources, but inorganic fluoride release was not stoichiometric. Metabolome studies revealed that this bacterium produces fluorinated anabolites and phospholipids. Mass spectrometric fatty acid profiling detected fluorinated long-chain (i.e., C-C) fatty acids in strain 273 cells grown with FD or DFD, and lipidomic profiling determined that 7.5 ± 0.2 and 82.0 ± 1.0% of the total phospholipids in strain 273 grown with FD or DFD, respectively, were fluorinated. The detection of the fluorinated metabolites and macromolecules represents a heretofore unrecognized sink for organofluorine, an observation with consequences for the environmental fate and transport of fluorinated aliphatic compounds.
人为产生的有机氟化合物具有抗降解性、全球分布性，对人类健康构成关注。尽管很少见，但自然过程会合成含氟化合物，而一些细菌已经进化出代谢有机氟化合物的机制。273 号菌株可以用 1-氟代癸烷（FD）和 1,10-二氟代癸烷（DFD）作为碳源生长，但无机氟化物的释放不是化学计量的。代谢组学研究表明，这种细菌会产生含氟的同化产物和磷脂。质谱脂肪酸分析检测到在 273 号菌株中用 FD 或 DFD 培养时，细胞中存在长链（即 C-C）含氟脂肪酸，脂质组学分析确定，用 FD 或 DFD 培养的 273 号菌株中，分别有 7.5±0.2%和 82.0±1.0%的总磷脂被氟化。这些含氟代谢物和大分子的检测代表了有机氟化合物的一个以前未被识别的汇，这一观察结果对氟化脂肪族化合物的环境命运和迁移具有影响。
