Department of Integrated Biological Science, Pusan National University, Busan, 46241, Republic of Korea; Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea.
Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University, Busan, 49104, Republic of Korea.
Fish Shellfish Immunol. 2022 Jul;126:178-186. doi: 10.1016/j.fsi.2022.05.043. Epub 2022 May 25.
MicroRNAs (miRNAs) are small non-coding RNAs that participate in various biological and cellular processes by regulating target gene expression. miRNAs are also known to play vital roles in the pathogenesis of various diseases, including infections, as well as the disease progression and defense responses. In this study, we examined the expression levels of pol-miR-140-3p and its target gene, kinesin family member 5A (KIF5A), in association with the Streptococcus parauberis (S. parauberis) infection, a major bacterial pathogen that causes streptococcosis in olive flounder (Paralichthys olivaceus). KIF5A is a heavy chain isoform of kinesin-1, which is known to be brain-specific, and this study is the first examination of KIF5A expression related to the regulation of miRNA in olive flounder (named PoKIF5A). There were significant differences in expression levels between infected and healthy olive flounder as the expression of pol-miR-140-3p in the infected fish was lower than that in the control, while the expression of PoKIF5A was higher in the infected fish than in the healthy controls. These contradictory results suggest that downregulated pol-miR-140-3p induces the expression of PoKIF5A against S. parauberis infection in olive flounder.
微小 RNA（miRNAs）是一类小的非编码 RNA，通过调节靶基因的表达参与各种生物和细胞过程。miRNAs 也被认为在各种疾病的发病机制中发挥着重要作用，包括感染，以及疾病的进展和防御反应。在这项研究中，我们检查了多瘤 miR-140-3p 及其靶基因驱动蛋白家族成员 5A（KIF5A）的表达水平，与链球菌副伯克霍尔德氏菌（S. parauberis）感染有关，S. parauberis 是一种主要的细菌病原体，可引起橄榄石斑鱼（Paralichthys olivaceus）的链球菌病。KIF5A 是驱动蛋白-1 的重链同工型，已知其在大脑中特异性表达，本研究首次检查了 KIF5A 表达与橄榄石斑鱼 miRNA 调控的关系（命名为 PoKIF5A）。感染和健康的橄榄石斑鱼之间的表达水平存在显著差异，因为感染鱼中的 pol-miR-140-3p 表达低于对照组，而感染鱼中的 PoKIF5A 表达高于健康对照组。这些矛盾的结果表明，下调的 pol-miR-140-3p 诱导了 PoKIF5A 的表达，以抵抗 S. parauberis 感染橄榄石斑鱼。
