Jo Ara, Im Jennifer, Lee Hee-Eun, Jang Dongmin, Nam Gyu-Hwi, Mishra Anshuman, Kim Woo-Jin, Kim Won, Cha Hee-Jae, Kim Heui-Soo
Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 46241, Republic of Korea; Genetic Engineering Institute, Pusan National University, Busan 46241, Republic of Korea.
Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 46241, Republic of Korea.
Gene. 2017 Sep 10;628:16-23. doi: 10.1016/j.gene.2017.07.020. Epub 2017 Jul 8.
MicroRNAs (miRNAs) are small non-coding RNAs (ncRNAs) that mainly bind to the seed sequences located within the 3' untranslated region (3' UTR) of target genes. They perform an important biological function as regulators of gene expression. Different genes can be regulated by the same miRNA, whilst different miRNAs can be regulated by the same genes. Here, the evolutionary conservation and expression pattern of miR-10a-3p in olive flounder and rock bream was examined. Binding sites (AAAUUC) to seed region of the 3' UTR of target genes were highly conserved in various species. The expression pattern of miR-10a-3p was ubiquitous in the examined tissues, whilst its expression level was decreased in gill tissues infected by viral hemorrhagic septicemia virus (VHSV) compared to the normal control. In the case of rock bream, the spleen, kidney, and liver tissues showed dominant expression levels of miR-10a-3p. Only the liver tissues in the rock bream samples infected by the iridovirus indicated a dominant miR-10a-3p expression. The gene ontology (GO) analysis of predicted target genes for miR-10a-3p revealed that multiple genes are related to binding activity, catalytic activity, cell components as well as cellular and metabolic process. Overall the results imply that the miR-10a-3p could be used as a biomarker to detect VHSV infection in olive flounder and iridovirus infection in rock bream. In addition, the data provides fundamental information for further study of the complex interaction between miR-10a-3p and gene expression.
微小RNA（miRNA）是一类小的非编码RNA（ncRNA），主要与位于靶基因3'非翻译区（3'UTR）内的种子序列结合。它们作为基因表达的调节因子发挥着重要的生物学功能。同一miRNA可调控不同基因，而不同的miRNA也可由相同基因调控。在此，研究了牙鲆和条石鲷中miR-10a-3p的进化保守性和表达模式。靶基因3'UTR种子区域的结合位点（AAAUUC）在不同物种中高度保守。miR-10a-3p的表达模式在所检测的组织中普遍存在，但其在感染病毒性出血性败血症病毒（VHSV）的鳃组织中的表达水平相对于正常对照有所下降。在条石鲷中，脾脏、肾脏和肝脏组织显示出miR-10a-3p的优势表达水平。在感染虹彩病毒的条石鲷样本中，只有肝脏组织显示出miR-10a-3p的优势表达。对miR-10a-3p预测靶基因的基因本体（GO）分析表明，多个基因与结合活性、催化活性、细胞成分以及细胞和代谢过程相关。总体而言，结果表明miR-10a-3p可作为检测牙鲆VHSV感染和条石鲷虹彩病毒感染的生物标志物。此外，这些数据为进一步研究miR-10a-3p与基因表达之间的复杂相互作用提供了基础信息。
