Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
J Glob Antimicrob Resist. 2022 Sep;30:451-458. doi: 10.1016/j.jgar.2022.05.014. Epub 2022 May 25.
The gut is a complex environment inhabited by a wide range of bacterial species. Lactobacillus species constitute a significant proportion of this environment and, due to their mobile genetic elements such as plasmids and transposons, are more likely to acquire and transfer antibiotic resistance genes through horizontal gene transfer (HGT).
The current study obtained and analysed 321 genome assemblies to determine the prevalence of intrinsic and acquired antibiotic resistance genes (ARGs) among Lactobacillus species colonizing the human gastrointestinal tract.
A total of four high-frequency resistance genes were identified, including dfra42 (42%), poxtA (17.4%), lmrB (12%), and BJP-1 (7.7%); aside from dfra42, which is an intrinsic resistance gene, the other genes are acquired resistance genes. PoxtA was found in several different species, mainly in L. paracasei, whereas BJP-1 and lmrB were found in only one species, L. rhamnosus. IS5-like elements family transposase flanked 11% and 8% of detected lmrB and BJP-1, respectively, while a variety of insertion sequences surrounded 22% of identified poxtA. Furthermore, to the best of our knowledge, this is the first report of BJP-1 in lactobacilli that would suggest it has transferred from soil microbiota to humans.
According to the 'One Health' perspective, early detection of a new reservoir would control the global spread of the antibiotic-resistant bacterial species among the three environments, which include humans, the environment, and animals. Finally, the study's findings may then highlight the possibility of lactobacilli acquiring or transmitting resistance to other species within or outside the human intestine.
肠道是一个复杂的环境，栖息着多种细菌。乳杆菌属构成了这个环境的重要组成部分，由于其移动遗传元件，如质粒和转座子，更容易通过水平基因转移（HGT）获得和转移抗生素耐药基因。
本研究获得并分析了 321 个基因组组装，以确定定植于人类胃肠道的乳杆菌属中固有和获得性抗生素耐药基因（ARGs）的流行情况。
共鉴定出 4 种高频耐药基因，包括 dfra42（42%）、poxtA（17.4%）、lmrB（12%）和 BJP-1（7.7%）；除了 dfra42 是固有耐药基因外，其他基因均为获得性耐药基因。PoxtA 存在于多种不同的物种中，主要存在于 L. paracasei 中，而 BJP-1 和 lmrB 仅存在于一种物种，即 L. rhamnosus 中。IS5 样元件家族转座酶分别侧翼包围了 11%和 8%的检测到的 lmrB 和 BJP-1，而各种插入序列则包围了 22%的识别出的 poxtA。此外，据我们所知，这是 BJP-1 在乳杆菌属中的首次报道，表明它已经从土壤微生物群转移到了人类体内。
根据“同一健康”的观点，早期发现新的耐药基因库将有助于控制三种环境（包括人类、环境和动物）中抗生素耐药细菌的全球传播。最后，研究结果可能突出了乳杆菌属在人类肠道内外获得或传播耐药性给其他物种的可能性。
