Kaewarsar Ekkachai, Chaiyasut Chaiyavat, Lailerd Narissara, Makhamrueang Netnapa, Peerajan Sartjin, Sirilun Sasithorn
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Foods. 2023 Oct 20;12(20):3847. doi: 10.3390/foods12203847.
The gut microbiota is a complex community of microorganisms that plays a vital role in maintaining overall health, and is comprised of and . The probiotic efficacy and safety of and for consumption were confirmed by experiments. The survival rate of the probiotics showed a significant decline in gut tract simulation; however, the survival rate was more than 50%. Also, the probiotics could adhere to Caco-2 cell lines by more than 90%, inhibit the pathogenic growths, deconjugate glycocholic acid and taurodeoxycholic acid through activity of bile salt hydrolase (BSH) proteins, and lower cholesterol levels by over 46%. Regarding safety assessment, and showed susceptibility to some antibiotics but resistance to vancomycin and were examined as γ-hemolytic strains. Anti-inflammatory properties of with Caco-2 epithelial cell lines showed the significantly highest value ( < 0.05) for interleukin-10. Furthermore, probiotics and prebiotics (inulin, fructooligosaccharides, and galactooligosaccharides) comprise synbiotics, which have potential effects on the increased abundance of beneficial microbiota, but do not affect the growth of harmful bacteria in feces samples. Moreover, the highest concentration of short chain fatty acid was of acetic acid, followed by propionic and butyric acid.
肠道微生物群是一个复杂的微生物群落，在维持整体健康方面起着至关重要的作用，它由[具体内容缺失]组成。[具体物质名称缺失]和[具体物质名称缺失]用于食用的益生菌功效和安全性通过[具体实验名称缺失]实验得到了证实。益生菌的存活率在肠道模拟环境中显著下降；然而，存活率仍超过50%。此外，益生菌能够以超过90%的比例黏附于Caco - 2细胞系，抑制病原菌生长，通过胆汁盐水解酶（BSH）蛋白的活性使甘氨胆酸和牛磺脱氧胆酸去结合，并使胆固醇水平降低超过46%。关于安全性评估，[具体物质名称缺失]和[具体物质名称缺失]对某些抗生素敏感，但对万古霉素耐药，并被检测为γ - 溶血菌株。[具体物质名称缺失]与Caco - 2上皮细胞系的抗炎特性显示白细胞介素 - 10的值显著最高（<0.05）。此外，益生菌和益生元（菊粉、低聚果糖和低聚半乳糖）组成合生元，它们对有益微生物群丰度的增加具有潜在影响，但不影响粪便样本中有害细菌的生长。而且，短链脂肪酸的最高浓度是乙酸，其次是丙酸和丁酸。
